Diaporthe/Phomopsis (D/P) is a complex of seed-borne fungi that severely affects soybean (Glycine max (L.) Merrill), one of the most important crops worldwide. Non-thermal plasma treatment is a fast, economic and ecological friendly technology that can destroy seed-borne fungi and improve seed quality. Soybean seeds were exposed for 1, 2 and 3 min to a quasi-stationary (50 Hz) dielectric barrier discharge plasma operating at atmospheric pressure air. Different carrying gases (O₂ and N₂) and barrier-insulating materials were used. In this work we focused on the ability of plasma to control D/P in soybean seeds and to enhance seed quality. To support these results, different antioxidant enzymes (catalase, superoxide dismutase and guaiacol peroxidase), lipid peroxidation and phytohormones (ABA and AIA) content in seeds were evaluated. The results demonstrated reductions of 29% in catalase activity and increments of 30% in glutathione content after plasma treatment, reversing the oxidative damage caused by D/P fungi. This eco-friendly technology improved soybean seed quality and, for the first time, its efficiency in controlling soybean seed-borne pathogen fungi that colonize the inside of seeds was demonstrated.

Diaporthe /拟茎点霉（d / P）是一个复杂的种子传播的真菌的，这严重影响了大豆（大豆（L.）Merrill），世界范围内最重要的作物之一。非热等离子体处理是一种快速，经济和生态友好的技术，可以破坏种子传播的真菌并提高种子质量。将大豆种子暴露于在大气压空气下运行的准静态（50 Hz）介电势垒放电等离子体中1分钟，2分钟和3分钟。不同的载气（O ₂和N ₂）和隔离层材料。在这项工作中，我们集中于血浆控制大豆种子中D / P和提高种子质量的能力。为了支持这些结果，评估了种子中不同的抗氧化酶（过氧化氢酶，超氧化物歧化酶和愈创木酚过氧化物酶），脂质过氧化和植物激素（ABA和AIA）含量。结果表明，血浆处理后过氧化氢酶活性降低了29％，谷胱甘肽含量增加了30％，从而逆转了由D / P真菌引起的氧化损伤。这项环保技术改善了大豆种子的质量，并首次证明了它在控制定居在种子内部的大豆种子传播的病原真菌方面的效率。