The objective of this study was to identify the S-nitrosylated proteins in aging samples of pork longissimus thoracis muscle (aged 0 and 3 d) and to study the effects of exogenous S-nitrosoglutathione (GSNO, concentration at 10 and 100 μM) treatments of aged 0 d sample. After validating modified biotin switch method, the samples were labeled with tandem mass tags (TMT126-129) for the LC-MS/MS analysis. A total of 366 peptides were identified to be S-nitrosylated corresponding to 339 proteins. Comparison of total intensity and individual S-nitrosylated sites between aging samples revealed that S-nitrosylation did occur in pork muscle during postmortem aging through possible pathways of denitrosylation and transnitrosylation. GSNO treatment groups showed a considerable number of potential cysteines could be modified with high thiol-reactivity. It was deduced that S-nitrosylation could be involved in the postmortem metabolic process possibly through the regulation of activity or function of glycolytic enzymes, calcium release, heat shock proteins, antioxidant enzymes and myofibrillar proteins.

这项研究的目的是鉴定猪肉长肌胸肌（年龄为0和3 d）的老化样品中的S-亚硝基化蛋白，并研究外源性S-亚硝基谷胱甘肽（GSNO，浓度为10和100μM）处理的效果。老化0 d样品。在验证改良的生物素转换方法后，样品用串联质量标签（TMT126-129）标记，用于LC-MS / MS分析。总共鉴定出366种肽被S-亚硝基化，对应于339种蛋白质。比较衰老样品之间的总强度和各个S-亚硝基化位点，发现在尸体衰老过程中，猪肉中的S-亚硝基化确实是通过脱亚硝化和反硝化的可能途径发生的。GSNO治疗组显示，大量的半胱氨酸可以被高硫醇反应性修饰。