This review discusses the integration of liquid chromatography (LC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) in the comprehensive analysis of small molecules from complex matrices. We first discuss the steps taken toward making the three technologies compatible, so as to create an efficient analytical platform. The development of online LC-MS-NMR, highlighted by successful applications in the profiling of highly concentrated analytes (LODs 10 μg) is discussed next. This is followed by a detailed overview of the alternative approaches that have been developed to overcome the challenges associated with online LC-MS-NMR that primarily stem from the inherently low sensitivity of NMR. These alternative approaches include the use of stop-flow LC-MS-NMR, loop collection of LC peaks, LC-MS-SPE-NMR, and offline NMR. The potential and limitations of all these approaches is discussed in the context of applications in various fields, including metabolomics and natural product discovery.

中文翻译：

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LC-MS和NMR的集成，可用于分析复杂基质中的低分子量痕量分析物。

这篇综述讨论了液相色谱法（LC），质谱法（MS）和核磁共振（NMR）在复杂基质中小分子的综合分析中的集成。我们首先讨论使三种技术兼容的步骤，以创建一个有效的分析平台。接下来将讨论在线LC-MS-NMR的开发，并着重介绍了在高浓度分析物（LODs 10μg）的分析中的成功应用。接下来是对为克服与在线LC-MS-NMR相关的挑战而开发的替代方法的详细概述，这些挑战主要源于NMR固有的低灵敏度。这些替代方法包括使用停止流LC-MS-NMR，LC峰的循环收集，LC-MS-SPE-NMR和离线NMR。