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Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products†
RSC Advances ( IF 3.9 ) Pub Date : 2018-07-19 00:00:00 , DOI: 10.1039/c8ra04460k
Ming Li 1 , Yuanyuan Zhang 1 , Rujin Zhao 2 , Zhenjiang Liu 1 , Xia Hong 1 , Yin Cui 1 , Yonglai Xue 1 , Daolin Du 1
Affiliation  

The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10), half-maximal inhibition concentration (IC50) and detection range (IC20–IC80) of BSA-IMB are 0.00579, 0.573 and 0.0183–17.9 ng mL−1, respectively, for AFB1. The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.

中文翻译:

基于免疫磁珠的生物素-链霉亲和素系统高效检测农产品中的黄曲霉毒素 B1†

使用免疫磁珠 (IMB) 的潜在均相测定已作为食品安全控制中的筛选工具而受到关注。该方法简单、高效,不需要较长的孵育时间或复杂的分离步骤。本研究成功开发了一种均相免疫测定法,并将IMB与生物素-链霉亲和素(BSA)(BSA-IMB)系统偶联,用于测定农产品中黄曲霉毒素B 1(AFB 1 )的污染。在最佳条件下,BSA-IMB的检测限(LOD,IC 10)、半数抑制浓度(IC 50)和检测范围(IC 20 –IC 80 )分别为0.00579、0.573和0.0183–17.9 ng mL -1,分别为 AFB 1。BSA-IMB检测AFB 1可在40分钟内完成(ELISA至少需要180分钟)。BSA-IMB 与其类似物的交叉反应可以忽略不计(<3.82%);这些结果表明选择性很高。农产品样品中 AFB 1的加标回收率范围为 89.6% 至 118.2%,相对标准偏差 (RSD) 为 3.4% 至 13.2% 。此外,真实样品的 BSA-IMB 结果显示可靠性和 0.9928 与 HPLC 荧光检测器的高度相关性。所提出的 BSA-IMB 系统被证明是在农产品样品的广泛检测范围内均质、高效、灵敏和替代检测 AFB 1的令人满意的工具。
更新日期:2018-07-19
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