Bioresource Technology ( IF 11.4 ) Pub Date : 2018-07-19 , DOI: 10.1016/j.biortech.2018.07.094 Mukesh Kumar Awasthi , Hongyu Chen , Quan Wang , Tao Liu , Yumin Duan , Sanjeev Kumar Awasthi , Xiuna Ren , Zhineng Tu , Jiao Li , Junchao Zhao , Zengqiang Zhang
In this study, the bacterial community succession and variations were investigated in poultry manure (PM) compost by the using high-throughput sequencing in six different concentration of clay [at 0% (T1), 2% (T2), 4% (T3), 6% (T4), 8% (T5) and 10% (T6) on PM dry weight basis] applied compost. The results indicated that dominant phylum were Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes, while Bacillus, Paenibacillus, Virgibacillus, Oceanobacillus and Clostridium were the dominant genera in all the treatments. Correlation analyses provided useful tools for insight into the bacterial interactions with environmental factors and also extension of the compost maturation and resistance of bacteria. During the course of study, the diversity of bacteria similar but relative abundance variable in each treatments. However, the average and the normalized (to bacterial RAs or copies of sequences) both remained greater in higher dosage of clay applied treatments. Finally, the RAs of various bacterial community composition was affected in PM compost by the clay application.
中文翻译:
与粘土混合堆肥的家禽粪便中细菌多样性的演替:其动力学及其与理化和气态参数的关联研究
在这项研究中,通过使用高通量测序对六种不同浓度的粘土[0%(T1),2%(T2),4%(T3) ),6%(T4),8%(T5)和10%(T6)(以PM干重计)]施用堆肥。结果表明,占主导地位的门是厚壁菌门,变形菌,放线菌和拟杆菌,而芽孢杆菌,芽孢杆菌,Virgibacillus,Oceanobacillus和梭状芽孢杆菌是所有治疗中的主要属。相关分析为深入了解细菌与环境因素之间的相互作用以及扩展堆肥成熟度和抵抗力提供了有用的工具。在研究过程中,每种处理中细菌的多样性相似但相对丰度不同。但是,在更高剂量的黏土处理中,平均值和归一化(针对细菌RA或序列副本)均保持较高水平。最后,黏土的施用会影响PM堆肥中各种细菌群落组成的RA。