Current estimates report that approximately 25% of U.S. adults use dietary supplements for medicinal purposes. Yet, regulation and transparency within the dietary supplement industry remains a challenge, and economic incentives encourage adulteration or augmentation of botanical dietary supplement products. Undisclosed changes to the dietary supplement composition could impact safety and efficacy; thus, there is a continued need to monitor possible botanical adulteration or mis-identification. Goldenseal, Hydrastis canadensis L. (Ranunculaceae), is a well-known botanical used to combat bacterial infections and digestive problems and is widely available as a dietary supplement. The goal of this study was to evaluate potential adulteration in commercial botanical products using untargeted metabolomics, with H. canadensis supplements serving as a test case. An untargeted ultraperformance liquid chromatography-mass spectrometry (LC-MS) metabolomics analysis was performed on 35 H. canadensis commercial products. Visual inspection of the chemometric data via principal component analysis (PCA) revealed several products that were distinct from the main groupings of samples, and subsequent evaluation of contributing metabolites led to their confirmation of the outliers as originating from a non-goldenseal species or a mixture of plant materials. The obtained results demonstrate the potential for untargeted metabolomics to discriminate between multiple unknown products and predict possible adulteration.

当前的估计报告指出，大约25％的美国成年人将膳食补充剂用于医疗目的。然而，膳食补充剂行业内的法规和透明度仍然是一个挑战，经济激励措施鼓励掺假或增加植物性膳食补充剂产品。膳食补充剂成分的未公开变化可能会影响安全性和功效；因此，仍然需要监视可能的植物掺假或错误标识。Goldenseal，Hydrastis canadensis L.（毛an科），是一种著名的植物药，用于对抗细菌感染和消化系统疾病，可作为膳食补充剂广泛使用。这项研究的目的是评估使用非靶向代谢组学在商业植物产品中的潜在掺假，以及H. canadensis补充剂充当测试用例。对35 H.canadensis商业产品进行了非靶向超高效液相色谱-质谱（LC-MS）代谢组学分析。通过主成分分析（PCA）目视检查化学计量数据，发现有几种产品与样品的主要组别不同，随后对代谢产物的评估导致他们确认了异常点来自非金毛种类或混合物。植物材料。获得的结果证明了无针对性的代谢组学有可能区分多种未知产物并预测可能的掺假。