In this study, it is aimed to investigate the antioxidant mechanism of new extracts from potatoes. Four pigments, namely, Petunin, Paeonin, Malvidin and Pelargonidin, were extracted from potatoes by high performance liquid chromatography (HPLC). Our results showed that the cellular morphology and cell viability were significantly altered in gastric mucosal epithelial cells (GES-1) treated with different hydrogen peroxide (H₂O₂) concentrations over time (P < 0.05). Paeonin presented the strongest anti-oxidative effects on H₂O₂-treated cells, in both a dose- and time-dependent manner, determined by ARE-luciferase activity and HO-1 mRNA expression. After pre-treatment with Paeonin in H₂O₂-exposed cells, Keap1, Nrf2, HO-1 and NQO1 protein expressions were remarkably up-regulated. Furthermore, immunostaining of Nrf2 expression was obviously elevated in the H₂O₂ + Paeonin group over time. The GSH content in the H₂O₂ + Paeonin group was notably lower than that in the H₂O₂ + Paeonin + GSK690693 group. Paeonin promoted cell cycle with augmented Cyclin D1 and p27 protein expressions. Moreover, Paeonin suppressed apoptosis with increased Bcl2, total Caspase3 and total Caspase8 protein expressions and decreased Bax, p-Caspase3 and p-Caspase8 protein expression in H₂O₂-treated cells. These results suggested that Paeonin might exert an anti-oxidative role by activating Nrf2 signaling pathway with the changes of cell cycle and apoptosis.

中文翻译：

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从马铃薯中提取的eon药通过PI3K / Akt介导的Nrf2信号通路在体外保护胃上皮细胞免受H2O2诱导的氧化损伤。

在这项研究中，目的是研究马铃薯新提取物的抗氧化机理。通过高效液相色谱法（HPLC）从马铃薯中提取了四种色素，即矮牵牛，eon药，Malvidin和Pelargonidin。我们的结果表明，随着时间的推移，不同浓度的过氧化氢（H ₂ O ₂）处理的胃粘膜上皮细胞（GES-1）的细胞形态和细胞活力均发生了显着变化（P <0.05）。通过ARE荧光素酶活性和HO-1 mRNA表达确定，药以剂量和时间依赖性方式对H ₂ O ₂处理的细胞表现出最强的抗氧化作用。在H ₂ O ₂中用Pa药进行预处理后暴露的细胞，Keap1，Nrf2，HO-1和NQO1蛋白表达显着上调。此外， 随着时间的推移，H ₂ O ₂ + Pa药组中Nrf2表达的免疫染色明显升高。H ₂ O ₂  + eon药组的GSH含量明显低于H ₂ O ₂  + eon药+ GSK690693组。eon药促进细胞周期蛋白D1和p27蛋白表达的增强。此外，Paeonin通过在H ₂ O _2中增加Bcl2，总Caspase3和总Caspase8蛋白表达并降低Bax，p-Caspase3和p-Caspase8蛋白表达来抑制细胞凋亡。处理的细胞。这些结果表明，eon药苷可能通过激活Nrf2信号通路并随着细胞周期和凋亡的改变而发挥抗氧化作用。