In this paper, we construct a fluorescence nucleic acid probe based on DNA-templated silver nanoclusters (DNA-Ag NCs) for the detection of the p53 gene. The fluorescence biosensing of the “turn-on” model is successfully implemented as a result of the target-triggered configurational change in the hairpin DNA probe and the synthesis of fluorescent Ag NCs. With this biosensor, the limit of detection (LOD) for the p53 gene is 3.57 nM and the linear range is 250–2500 nM in phosphate buffer solution, while a LOD of 6.06 nM in the linear range of 250–2500 nM is obtained in 1% diluted fetal calf serum. So this probe, with its advantages of specificity, practical application, easy operation and low cost, will have favourable development prospects in biological sensing and imaging.

中文翻译：

---

基于 DNA 模板化银纳米簇的核酸探针用于肿瘤抑制基因 p53 的开启荧光检测†

在本文中，我们构建了一种基于 DNA 模板化银纳米簇 (DNA-Ag NCs) 的荧光核酸探针，用于检测 p53 基因。“开启”模型的荧光生物传感由于发夹 DNA 探针中目标触发的构型变化和荧光 Ag NCs 的合成而成功实现。使用这种生物传感器，p53 基因在磷酸盐缓冲溶液中的检测限 (LOD) 为 3.57 nM，线性范围为 250-2500 nM，而在 250-2500 nM 的线性范围内，检测限为 6.06 nM。 1% 稀释的胎牛血清。因此，该探针以其特异性、实用性强、操作简便、成本低廉等优点，将在生物传感和成像方面具有良好的发展前景。