Disinfection byproducts (DBPs) are produced during the disinfection of drinking water and pose a hazard to human health. As a typical type of DBPs, iodoacetic acid (IAA) exhibits prominent cytotoxicity in mammalian cell systems which links with oxidative stress. However, little is known about the relationship of catalase (CAT) with the cytotoxicity of IAA and the adverse effects of IAA to CAT. This study investigated the effects of IAA on the cell viability and CAT activity in the mouse primary hepatocytes. It was shown that IAA exposure induced the loss of cell viability and the increase of intracellular CAT activity. Intracellular CAT activity significantly increased due to the stimulation of CAT production under IAA exposure. The molecular CAT activity was inhibited due to the direct interaction of IAA with HIS 74 and TYR 357 around the active sites of CAT. IAA binds to CAT with (4.05 ± 1.98) sites via van der Waals and hydrogen bonding interactions, resulting in the loosening of protein skeletons and the change of protein size.

饮用水消毒过程中会产生消毒副产物（DBP），对人体健康构成危害。作为典型的DBP类型，碘乙酸（IAA）在哺乳动物细胞系统中表现出明显的细胞毒性，这与氧化应激有关。但是，关于过氧化氢酶（CAT）与IAA的细胞毒性以及IAA对CAT的不良影响之间的关系知之甚少。这项研究调查了IAA对小鼠原代肝细胞中细胞活力和CAT活性的影响。结果表明，IAA暴露引起细胞活力的丧失和细胞内CAT活性的增加。在IAA暴露下，细胞内CAT活性由于刺激CAT产生而显着增加。由于IAA与CAT活性位点周围的HIS 74和TYR 357直接相互作用，因此抑制了分子​​CAT的活性。IAA通过范德华力和氢键相互作用与（4.05±1.98）位的CAT结合，导致蛋白质骨架的松弛和蛋白质大小的变化。