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Stability and degradation of indocyanine green in plasma, aqueous solution and whole blood†
Photochemical & Photobiological Sciences ( IF 3.1 ) Pub Date : 2018-07-17 00:00:00 , DOI: 10.1039/c8pp00064f
Sonani Mindt 1, 2, 3, 4, 5 , Ioannis Karampinis 2, 3, 4, 5, 6 , Michael John 5, 7, 8, 9 , Michael Neumaier 1, 2, 3, 4, 5 , Kai Nowak 2, 3, 4, 5, 6
Affiliation  

Fluorescence-based imaging has evolved into an important technology during recent years. Specifically indocyanine green (ICG) has invaded most fields of diagnostic and interventional medicine. Considering the numerous advantages of the substance like the rapid degradation and rare adverse reactions, ICG is currently the most commonly used fluorescing agent. High-performance liquid chromatography (HPLC) was used for measuring absorbance and fluorescence of ICG and its potential degradation compounds. Stability and degradation were evaluated under light exposure or in darkness at various temperatures. Under these conditions, degradation of ICG was evaluated over a period of 11 days. Additional, stability measurements of ICG were performed in EDTA whole blood samples at 37 °C incubation temperature while monitoring. Furthermore, we used mass spectrometric (MS) and nuclear magnetic resonance (NMR) analyses for the identification of supposed ICG degradation compound. Potential quenching effect of ICG was examined in aqueous and plasma solutions at concentrations ranging from 0.01–100 μg ml−1. When diluted in water and stored at 4 °C in the dark, ICG is stable for three days with only 20% of fluorescence intensity lost in this time-span. ICG incubated at 37 °C in whole blood under light exposure is stable for 5 h. In our study we observed the degradation of ICG into two degradation compounds with a mass of m/z 785.32 and m/z 1501.57, respectively. Based on our observations we suggest that ICG should be used within one or two days after preparation, if the ICG solution is stored at 4 °C.

中文翻译:

血浆,水溶液和全血中吲哚菁绿的稳定性和降解

近年来,基于荧光的成像已发展成为一项重要技术。特别是吲哚菁绿(ICG)已侵入诊断和介入医学的大多数领域。考虑到该物质具有许多优点,例如快速降解和罕见的不良反应,因此,ICG是目前最常用的荧光剂。高效液相色谱(HPLC)用于测量ICG及其潜在降解化合物的吸光度和荧光。在曝光或黑暗中在不同温度下评估稳定性和降解。在这些条件下,在11天的时间内评估了ICG的降解。另外,在EDTA全血样品中,在37°C孵育温度下进行ICG稳定性测量,同时进行监测。此外,我们使用质谱(MS)和核磁共振(NMR)分析来鉴定假定的ICG降解化合物。在浓度为0.01–100μgml的水溶液和血浆溶液中检查了ICG的潜在淬灭作用-1。当用水稀释并在黑暗中于4°C储存时,ICG可以稳定三天,在这段时间内仅损失20%的荧光强度。在37°C下于全血中于光照下孵育的ICG稳定5小时。在我们的研究中,我们观察到ICG降解为质量分别为m / z 785.32和m / z 1501.57的两种降解化合物。根据我们的观察,我们建议,如果ICG溶液在4°C下储存,则应在制备后的一两天内使用ICG。
更新日期:2018-07-17
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