Determining the migratory and invasive capacity of cancer cells as well as clarifying the underlying mechanisms are most relevant for developing biosensors in cancer diagnosis, prognosis, drug development and treatment. Intravasation of metastatic cells into blood stream initiated by their invasion to vascular layer would be a significant characteristic of metastasis. Many types of biochemical and bioelectrical sensors were developed for early detection of metastasis. The simplicity of the setup, the ease of the readout, detection of the trace of rare metastatic cells and the feasibility to perform the assay with standard laboratory equipment are some of the challenges limiting the usability of the sensors in tracing the metastasis. Here we describe a biosensor based on recently reported metastatic diagnosis assay; Metas-Chip, with the assistance of nanoroughened Poly-methyl methacrylate (PMMA) layer to diagnose populated metastatic breast cells from primary cancerous ones. Retraction and detachment of Human Umbilical Vein Endothelial Cells (HUVECs) invaded by metastatic cells as a recently found phenomena is the mechanism of the action. A population of HUVECs would be detached from the gold microelectrodes, patterned on nanoroughened surface, which would lead to large changes in impedance. Here, applying biocompatible and patternable nanoroughened surface instead of using adhesive layers which might produce electrical noises resulted in great sensitivity and detectivity of the sensor. Apart from the tight interaction between endothelial cells and nanocontacts of the electrodes, using low concentration (10%) of tumor cells in this invasion assay, might enhance its application in clinical trials.

确定癌细胞的迁移和侵袭能力以及阐明其潜在机制与开发生物传感器用于癌症的诊断，预后，药物开发和治疗最相关。由转移细胞侵入血管层引起的转移细胞向血管内的浸润将是转移的重要特征。开发了多种类型的生化和生物电传感器以用于转移的早期检测。设置的简单性，读取的简便性，稀有转移细胞痕迹的检测以及使用标准实验室设备进行测定的可行性是限制传感器追踪转移的可用性的一些挑战。在这里，我们根据最近报道的转移性诊断分析描述一种生物传感器；元芯片 在纳米粗糙的聚甲基丙烯酸甲酯（PMMA）层的辅助下，可以诊断出原发癌的人口转移性乳腺细胞。作为最近发现的现象，转移细胞侵袭的人脐静脉内皮细胞（HUVEC）的回缩和脱离是其作用机理。大量的HUVEC将与金微电极分离，并在纳米粗糙化的表面上进行构图，这将导致阻抗发生较大变化。在这里，应用生物相容性和可图案化的纳米粗糙表面，而不是使用可能会产生电噪声的粘合剂层，会导致传感器具有很高的灵敏度和可检测性。除了内皮细胞和电极的纳米触点之间的紧密相互作用外，在这种侵袭试验中使用低浓度（10％）的肿瘤细胞，