Tyramine, an important biogenic amine, occurs in high amounts in certain processed food products and can cause adverse health effects in people. In this study, we developed a simple method to produce a molecularly imprinted fluorescence sensor with high sensitivity and selectivity for tyramine. The reverse microemulsion method was applied to prepare a carrier material, hydrophobic CdSe/ZnS quantum dots (QDs) sensitized with carboxylated graphene (Gra), which was added to the reaction for synthesizing molecularly imprinted polymers (MIPs) using 3-mercaptopropyltriethoxysilane (MPTES) as the functional monomer and tetraethylorthosilicate (TEOS) as the crosslinking agent in the presence of tyramine as the target molecule. The novel sensing material, Gra-QDs@MIPs, was selective for tyramine and generated a concentration-dependent fluorescence signal. Under optimized conditions, the limit of detection was 0.021 mg L⁻¹ and the linear range of detection was 0.07–12 mg L⁻¹. Importantly, we tested the performance of the Gra-QDs@MIPs with real-world samples by demonstrating that they selectively detected around 0.08 or 2 mg L⁻¹ of tyramine in spiked rice wine samples. Hence, the described molecularly imprinted fluorescence sensor for tyramine could serve as a prototype for developing MIP-based assays for food analysis.

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基于量子点石墨烯和分子印迹技术的黄酒中酪胺检测新方法

酪胺是一种重要的生物胺，在某些加工食品中大量存在，并可能对人类造成不利的健康影响。在这项研究中，我们开发了一种简单的方法来生产对酪胺具有高灵敏度和选择性的分子印迹荧光传感器。运用反向微乳液法制备了载体材料，用羧化石墨烯（Gra）敏化的疏水性CdSe / ZnS量子点（QDs），将其加入到使用3-巯基丙基三乙氧基硅烷（MPTES）合成分子印迹聚合物（MIPs）的反应中在酪胺作为目标分子的情况下，作为功能性单体的四氟乙烯和作为交联剂的原硅酸四乙酯（TEOS）。新型传感材料Gra-QDs @ MIPs对酪胺具有选择性，并产生浓度依赖性的荧光信号。^-1和线性检测范围是0.07-12 mg L ^-1。重要的是，我们通过证明它们在加标的米酒样品中选择性检测到约0.08或2 mg L ^-1的酪胺来测试真实样品的Gra-QDs @ MIPs的性能。因此，所描述的用于酪胺的分子印迹荧光传感器可以用作开发用于食品分析的基于MIP的测定的原型。