Fourier Transform Infrared (FTIR) microspectroscopy was used to highlight the interactions between two photosensitisers (PS) of different geometries, TPPmOH4 and a glycoconjugated analogous, TPPDegMan, and lipid bilayers modelling retinoblastoma cell membranes. Retinoblastoma is a rare disease occurring in young infants, for whom conservative treatments may present harmful side-effects. Photodynamic therapy (PDT) is expected to induce less side-effects, as the photosensitiser is only activated when the tumour is illuminated.

Since efficiency of the treatment relies on photosensitiser penetration in cancer cells, bilayers with three lipid compositions - pure SOPC, SOPC/SOPE/SOPS/Chol (56:23:11:10) and SOPC/SOPE/SOPS/Chol/CL (42:32:9:8:6) – were used as plasma and mitochondria model membranes. FTIR spectra showed that the interaction of the PSs with the lipid bilayers impacted the lipid organization of the latter, causing significant spectral variations. Both studied photosensitisers inserted at the level of lipid hydrophobic chains, increasing chain fluidity and disorder. This was confirmed by surface pressure measurements. Photosensitisers - TPPmOH4 more than TPPDegMan - also interacted with the polar region of the bilayer, forming hydrogen bonds with phosphate groups that induced major shifts of phosphate absorption bands. This difference in PS interaction with moieties in the polar region was more pronounced with the models with complex lipid composition.

使用傅立叶变换红外（FTIR）显微技术来突出显示两个不同几何形状的光敏剂（PS）TPPmOH4与糖缀合的类似物TPPDegMan和模拟视网膜母细胞瘤细胞膜的脂质双层之间的相互作用。视网膜母细胞瘤是一种罕见的疾病，发生在幼儿中，保守治疗可能会带来有害的副作用。由于光敏剂仅在肿瘤被照亮时才被激活，因此光动力疗法（PDT）有望引起较少的副作用。

由于治疗的效率取决于光敏剂在癌细胞中的渗透，因此具有三种脂质成分的双层-纯SOPC，SOPC / SOPE / SOPS / Chol（56：23：11：10）和SOPC / SOPE / SOPS / Chol / CL（42 ：32：9：8：6）–用作血浆和线粒体模型膜。FTIR光谱表明PS与脂质双层的相互作用影响了后者的脂质组织，从而引起了明显的光谱变化。两者都研究了在脂质疏水链水平上插入的光敏剂，增加了链的流动性和无序性。通过表面压力测量证实了这一点。光敏剂-TPPmOH4比TPPDegMan多-还与双层的极性区域相互作用，与磷酸基团形成氢键，引起磷酸根吸收带的主要移动。在具有复杂脂质组成的模型中，PS与极性区域部分相互作用的差异更加明显。