An aptamer-based microchip capillary electrophoresis coupled with laser induced fluorescence (MCE-LIF) detection method for fast determination of Aflatoxin B₁ (AFB₁) and Ochratoxin A (OTA) was developed. Aptamers that are specific to these two mycotoxins were first hybridized with their aptamer complementary oligonucleotides. The double strand DNA that comes in contact with mycotoxin-containing environment would be unwound into separate aptamer-mycotoxin complex and aptamer complementary single strand. Different types of oligonucleotides can be separated in MCE and detected under the aid of fluorescent dye SYBR gold in LIF detection unit. Under the optimal conditions, on-chip aptamer-mycotoxin conjugates analysis was achieved within 3 min with extremely low LODs (0.026 ng/mL for AFB₁ and 0.021 ng/mL for OTA). Specificity study indicated that other major mycotoxins would not cross-react with these two aptamers, demonstrating the good selectivity of the proposed method. Quantification of trace AFB₁ and OTA in real food samples was carried out and satisfactory recoveries were obtained. It is demonstrated that this method is fast, facile and specific for Simultaneous determination of trace AFB₁ and OTA from foodstuffs.

建立了基于适配子的微芯片毛细管电泳结合激光诱导荧光（MCE-LIF）检测方法，用于快速测定黄曲霉毒素B ₁（AFB ₁）和O曲毒素A（OTA）的方法。首先，将对这两种霉菌毒素具有特异性的适体与它们的适体互补寡核苷酸杂交。与含有霉菌毒素的环境接触的双链DNA将解开成单独的适体-霉菌毒素复合物和适体互补单链。可以在MCE中分离不同类型的寡核苷酸，并借助LIF检测单元中的荧光染料SYBR gold进行检测。在最佳条件下，可在3分钟内以极低的LOD（AFB为0.026 ng / mL）完成芯片上适体-霉菌毒素结合物分析₁和0.021 ng / mL（对于OTA）。特异性研究表明，其他主要真菌毒素不会与这两种适体发生交叉反应，证明了所提出方法的良好选择性。对真实食品样品中的痕量AFB ₁和OTA进行了定量，回收率令人满意。结果表明，该方法快速，简便，特异性强，可同时测定食品中的痕量AFB ₁和OTA。