The current study describes quantitatively the interactions between (a) activity of extracellular enzymes: β‐glucosidase (BG), protease (Prot), alkaline phosphatase (Alk P), and acid phosphatase (Acid P); (b) intracellular activities: respiration, dehydrogenase activity (DHA); and (c) C and N contents of soil: C_org—organic carbon, C_mic—microbial biomass, ratio C_mic:C_org, N_tot—total nitrogen, and ratio C:N in earthworm‐affected and surrounding loess soil under a pear orchard. The soil tested was collected from: burrow walls (0–3 mm) and a transition zone (3–7 mm) both forming drilosphere, bulk soil (20 mm from the burrow wall), and cast aggregates. The enzyme activities were presented as total ‘tot’ (per gram of dry soil mass) and specific ‘spec’ (per C_mic). It was shown that soil C_mic, basal respiration, BG_tot, Prot_tot, and Alk P_tot were significantly positively correlated with C_org, C_mic:C_org, N_tot, and C:N (r = 0.627–0.976). However, the specific BG_spec, Prot_spec, and Acid P_spec were negatively correlated, while specific respiration (qCO₂) and Alk P_spec were not correlated with most soil characteristics. The relationships between DHA_tot, DHA_spec, and all soil characteristics were positive and had the highest correlation coefficients (r = 0.703–0.989). Response ratio of the enzyme activity was more strongly affected by enzyme activity location (extracellular vs. intracellular) and presentation method (total vs. specific), than by earthworm‐affected soil compartments. The results suggest a need to consider the method of enzyme activity presentation (specific vs. total) that supports understanding ecologically relevant microbial processes in earthworm‐inhabited orchard soils.

中文翻译：

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worm侵害的梨园土壤总酶和比酶活性与碳，氮含量之间的定量相互作用

当前的研究定量描述了（a）细胞外酶活性之间的相互作用：β-葡萄糖苷酶（BG），蛋白酶（Prot），碱性磷酸酶（Alk P）和酸性磷酸酶（Acid P）；（b）细胞内活性：呼吸，脱氢酶活性（DHA）；C：土壤和（c）C，N含量_的有机-有机碳，C_麦克风-microbial生物量，比率C _MIC：C_组织，N _TOT—梨园下earth和周围的黄土土壤中的总氮和碳氮比（C：N）。测试的土壤是从：形成壁圈的洞穴壁（0–3 mm）和过渡带（3–7 mm），块状土壤（距洞穴壁20 mm）和浇筑的集料中收集的。酶的活性呈现为总“TOT”（每克干土体）和比“规范”（的每个C _MIC）。结果表明，土壤C _mic，基础呼吸，BG _tot，Prot _tot和Alk P _tot与C _org，C _mic：C _org，N _tot和C：N呈显着正相关（r  = 0.627-0.976）。但是，具体的BG_spec，Prot_规范和Acid P_规范呈负相关，而比呼吸（qCO ₂）和Alk P_规范与大多数土壤特性无关。DHA _tot，DHA_规格与所有土壤特性之间的关系是正的，并且具有最大的相关系数（r = 0.703–0.989）。与受activity影响的土壤区室相比，酶活性的响应率受酶活性位置（细胞外与细胞内）和呈递方法（总与特异性）的影响更大。结果表明，需要考虑酶活表达的方法（特异性还是总量），以支持了解earth果园土壤中与生态相关的微生物过程。