Novel bone growth‐stimulating interfaces are designed via surface modification of titanium (Ti) surfaces using the bioceramic CaCO₃ in the vaterite phase, Ca‐crosslinked alginate hydrogel, or a blend of these two materials with an active enzyme, alkaline phosphatase (ALP), as an osteoinductive component. The surface morphology and chemistry of the engineered surfaces are investigated using scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy, while the vaterite crystal fraction within the inorganic phase of the different coating types is determined by X‐ray diffraction. The functionality of the osteoconductive assembled bioceramic–hydrogel interface on Ti surface in regard with an active ALP payload is verified by the surface ALP loading and its activity. The methods of loading of ALP onto a Ti surface, adsorption versus coprecipitation, have a significant influence on the activity of immobilized ALP amount. The osteoblasts cultivated on the engineered surfaces functionalized with ALP exhibit a higher viability. The proposed composite materials with an active surface and a high mineral content represent an attractive biointerface for tissue engineering.

中文翻译：

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基于生物陶瓷碳酸钙/水凝胶涂层的钛与活性酶刺激成骨细胞生长的纳米结构生物界面。

通过使用生物陶瓷CaCO ₃对钛（Ti）表面进行表面修饰，设计了新型的骨骼生长刺激界面。在球ate石相中，钙交联的藻酸盐水凝胶，或这两种材料与活性酶碱性磷酸酶（ALP）的混合物，作为骨诱导成分。使用扫描电子显微镜，原子力显微镜和傅立叶变换红外光谱法研究了工程表面的表面形态和化学性质，而不同涂层类型的无机相中的球ate石晶体分数则通过X射线衍射确定。钛表面上的骨传导性组装生物陶瓷-水凝胶界面的功能与活性ALP有效负载有关，已通过表面ALP负载及其活性得到验证。将ALP加载到Ti表面的方法，吸附与共沉淀的方法，对固定化ALP量的活性有重大影响。在用ALP功能化的工程化表面上培养的成骨细胞显示出更高的生存能力。所提出的具有活性表面和高矿物质含量的复合材料代表了用于组织工程的有吸引力的生物界面。