Abstract In this study, we report a novel feasible single step reduction method for the active synthesis of biocompatible chitosan coated copper oxide nanoparticles (Cs-CuO NPs), for loading and delivery of doxorubicin (DOX) into breast cancer cells. The formulated nanoparticles (NPs) were characterized by size, shape and surface charge using various sophisticated instruments. Interestingly, these formulated particles showed profound drug loading activity and also showed its versatile nature of DOX release at different pH which makes them as ideal particles for breast cancer therapy. Intracellular uptake was evaluated through fluorescence microscopy studies with the help of coumarin. Morphological and apoptotic studies with the DOX-Cs-CuO NPs on MCF-7 cells showed its ability to induce apoptosis in breast cancer cells, which was clearly substantiated by ROS generation with the loss of mitochondrial membrane potential. Our results clearly showed the therapeutic nature of novel biocompatible Cs-CuO NPs in delivering DOX with a pH-dependent manner.

中文翻译：

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纳米结构 pH 响应生物相容性壳聚糖包覆氧化铜纳米颗粒：一种用于乳腺癌细胞中多柔比星的聚合物智能细胞内递送系统

摘要 在这项研究中，我们报告了一种新的可行的单步还原方法，用于主动合成生物相容性壳聚糖包覆的氧化铜纳米粒子 (Cs-CuO NPs)，将阿霉素 (DOX) 加载和递送到乳腺癌细胞中。使用各种精密仪器对配制的纳米颗粒 (NP) 进行尺寸、形状和表面电荷的表征。有趣的是，这些配制的颗粒显示出强大的载药活性，并且还显示出其在不同 pH 值下释放 DOX 的多功能特性，这使它们成为乳腺癌治疗的理想颗粒。在香豆素的帮助下，通过荧光显微镜研究评估细胞内摄取。DOX-Cs-CuO NPs 对 MCF-7 细胞的形态学和细胞凋亡研究表明其具有诱导乳腺癌细胞凋亡的能力，随着线粒体膜电位的丧失，ROS的产生清楚地证实了这一点。我们的结果清楚地表明了新型生物相容性 Cs-CuO NPs 在以 pH 依赖性方式递送 DOX 方面的治疗性质。