Mass spectrometry (MS)-based methods for analyzing protein higher order structures have gained increasing application in the field of biopharmaceutical development. The predominant methods used in this area include native MS, hydrogen deuterium exchange-MS, covalent labeling, cross-linking and limited proteolysis. These MS-based methods will be briefly described in this article, followed by a discussion on how these methods contribute at different stages of discovery and development of protein therapeutics.

中文翻译：

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基于 MS 的重组蛋白构象分析在生物药物设计、优化和开发中的应用

基于质谱 (MS) 的蛋白质高级结构分析方法在生物制药开发领域得到了越来越多的应用。该领域使用的主要方法包括天然质谱、氢氘交换质谱、共价标记、交联和有限的蛋白水解。本文将简要介绍这些基于 MS 的方法，然后讨论这些方法如何在蛋白质疗法的不同发现和开发阶段发挥作用。