Native mass spectrometry (MS) is an emerging approach for characterizing biomacromolecular structure and interactions under physiologically relevant conditions. In native MS measurement, intact macromolecules or macromolecular complexes are directly ionized from a non-denaturing solvent, and key noncovalent interactions that hold the complexes together can be preserved for MS analysis in the gas phase. This technique provides unique multi-level structural information such as conformational changes, stoichiometry, topology and dynamics, complementing conventional biophysical techniques. Despite the maturation of native MS and greatly expanded range of applications in recent decades, further dissemination is needed to make the community aware of such a technique. In this review, we attempt to provide an overview of the current body of knowledge regarding major aspects of native MS and explain how such technique contributes to the characterization of biomacromolecular higher-order structure and interactions.

中文翻译：

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原生质谱如何有助于表征生物大分子的高阶结构和相互作用？

原生质谱 (MS) 是一种新兴方法，用于表征生理相关条件下的生物大分子结构和相互作用。在原始 MS 测量中，完整的大分子或大分子复合物直接从非变性溶剂中电离，并且可以保留将复合物结合在一起的关键非共价相互作用，以便在气相中进行 MS 分析。该技术提供了独特的多层次结构信息，如构象变化、化学计量、拓扑和动力学，补充了传统的生物物理技术。尽管近几十年来本地 MS 已经成熟并大大扩展了应用范围，但仍需要进一步传播以使社区了解这种技术。在这次审查中，