In the interest of developing novel electrocatalyst for high performance DNA biosensing, with distinctive chiral double helix nanostructure, nitrogen doped chiral carbonaceous nanotube (Chiral-CNT) was employed for ultrasensitive label-free DNA biosensing research. Chiral-CNT can quantitative detection of four DNA bases with high sensitivity and selectivity. Without any prehydrolysis and labeling process, direct electrochemistry of single-stranded DNA and double-stranded DNA, qualitative and quantitative detection of DNA hybridization (low detection limit: 0.0268 g L-1) were realized. Moreover, sensitive detection of DNA damage induced by fenton reagent was also realized with low detection limit of 0.0350 mg mL-1 and high sensitivity of 7.42 μA mg-1 mL. The high biosensing performance attributes to the unique chiral structure of Chiral-CNT, leads to efficient interreaction between Chiral-CNT and DNA molecule.

中文翻译：

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用于超灵敏 DNA 直接电化学、DNA 杂交和损伤研究的氮掺杂手性碳纳米管

为了开发用于高性能 DNA 生物传感的新型电催化剂，具有独特的手性双螺旋纳米结构，氮掺杂手性碳纳米管 (Chiral-CNT) 被用于超灵敏无标记 DNA 生物传感研究。手性碳纳米管可以对四种DNA碱基进行定量检测，灵敏度和选择性都很高。无需任何预水解和标记过程，实现了单链DNA和双链DNA的直接电化学，DNA杂交的定性和定量检测（检测下限：0.0268 g L-1）。此外，还实现了对芬顿试剂诱导的DNA损伤的灵敏检测，检测下限为0.0350 mg mL-1，灵敏度高为7.42 μA mg-1 mL。高生物传感性能归因于手性碳纳米管独特的手性结构，