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Short communication: Occurrence of methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci in dairy goat herds in Ohio, United States
Journal of Dairy Science ( IF 3.5 ) Pub Date : 2018-07-12 , DOI: 10.3168/jds.2017-13361
G.S. Moura , W.A. Gebreyes , M.F.S. Marques , D.T. Stipp , F.N. Souza , L.B. Da Costa , C.J.B. Oliveira

In light of the scarcity of information about the occurrence and epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MRCNS) in small ruminants in general, and particularly dairy goats, we launched this limited-scope study. The findings reported here show the detection of MRSA and MRCNS in goat milk and teat skin samples from dairy goat herds in the state of Ohio. A total of 120 milk samples and 120 teat-swab samples were collected from 5 farms. After conventional isolation and phenotypic characterization of the staphylococci colonies, bacterial isolates were tested by PCR assay targeting the genes nuc to identify Staphylococcus aureus and mecA to detect MRSA and MRCNS. The clonal complexes of MRSA isolates was also determined by multiloccus sequence typing. Fifteen (6.2%) positive S. aureus samples were found in this study: 9 from milk and 6 from teat skin samples. Four (2%) MRSA isolates were detected and, using multiloccus sequence typing genotyping, these were designated to clonal complexes CC133 (n = 2; milk samples) and CC5 (n = 2; teat skin). Three (1.25%) coagulase-negative staphylococci isolates from the teat skin also harbored the mecA gene. Although, the MRSA isolated from milk samples is not a typical human-associated lineage, the CC5 clone isolated from teat skin is a common and widespread clonal complex associated with humans, suggesting that this extramammary niche could be a relevant reservoir of methicillin-resistant staphylococci. Furthermore, the fact that 75% of MRSA were recovered from 1 farm showing poor hygiene practices strengthens the hypothesis that good hygiene practices could be useful to prevent persistence and spread of MRSA at a farm level.



中文翻译:

简短交流:美国俄亥俄州奶山羊群中耐甲氧西林金黄色葡萄球菌和凝固酶阴性葡萄球菌的发生

鉴于一般小反刍动物,特别是奶山羊中,关于耐甲氧西林的金黄色葡萄球菌(MRSA)和凝固酶阴性葡萄球菌(MRCNS)的发生和流行病学信息的匮乏,我们开展了这项有限范围的研究。此处报道的发现表明,在俄亥俄州的山羊奶和奶头皮肤样本中检测到了MRSA和MRCNS。从5个农场中总共收集了120个牛奶样品和120个奶头拭子样品。在常规分离葡萄球菌菌落并进行表型鉴定后,通过针对nuc基因的PCR分析检测细菌分离株,以鉴定金黄色葡萄球菌mecA检测MRSA和MRCNS。还通过多基因座序列分型确定了MRSA分离株的克隆复合物。在这项研究中发现了15份(6.2%)阳性的金黄色葡萄球菌样品:9份来自牛奶,6份来自乳头皮肤样品。检测到四个(2%)MRSA分离株,并使用多基因座序列分型基因分型法,将它们指定为克隆复合物CC133(n = 2;牛奶样品)和CC5(n = 2;乳头皮肤)。来自乳头皮肤的三种(1.25%)凝固酶阴性葡萄球菌分离株也带有mecA基因。尽管从牛奶样品中分离出的MRSA不是典型的人类相关谱系,但从乳头皮肤中分离出的CC5克隆是与人类相关的常见且广泛的克隆复合体,这表明这种乳腺外生态位可能是耐甲氧西林葡萄球菌的重要贮藏库。 。此外,从1个农场中回收了75%的MRSA的事实表明,其不良的卫生习惯强化了以下假设:良好的卫生习惯可能有助于防止MRSA在农场一级的持久性和传播。

更新日期:2018-07-14
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