A family of dipeptidyl enoates has been prepared and tested against the parasitic cysteine proteases rhodesain, cruzain and falcipain-2 related to sleeping sickness, Chagas disease and malaria, respectively. They have also been tested against human cathepsins B and L1 for selectivity. Dipeptidyl enoates resulted to be irreversible inhibitors of these enzymes. Some of the members of the family are very potent inhibitors of parasitic cysteine proteases displaying k_2nd (M⁻¹s⁻¹) values of seven orders of magnitude. In vivo antiprotozoal testing was also performed. Inhibitors exhibited IC₅₀ values in the micromolar range against Plasmodium falciparum, Trypanosoma brucei, Trypanosoma cruzi and even more promising lower values against Leishmania donovanii.

已经制备了二肽烯醇酯家族，并针对与睡眠病，恰加斯病和疟疾有关的寄生性半胱氨酸蛋白酶罗氏蛋白酶，克鲁萨因和falcipain-2进行了测试。还针对人组织蛋白酶B和L1对它们进行了选择性测试。二肽烯醇酸酯是这些酶的不可逆抑制剂。该家族中的某些成员是非常有效的寄生半胱氨酸蛋白酶抑制剂，显示出7个数量级的k _2nd（M ^-1 s ^-1）值。还进行了体内抗原生动物测试。抑制剂对恶性疟原虫，布鲁氏锥虫，克鲁氏锥虫的微摩尔范围显示IC ₅₀值甚至对利什曼原虫（Leishmania donovanii）的价值更低。