We developed a facile method for enhancing the sensitivity of lateral flow immunoassays (LFIAs) using core–shell hybrid nanofibers comprising a water-soluble polymer in the shell and silver-reducing reagents in the core. These nanofibers were produced using a coaxial electrospinning method and directly deposited in front of the test line on a nitrocellulose (NC) membrane. When a human serum spiked with various concentrations of Troponin I (TnI), a cardiac biomarker, was dropped on the absorption pad, a color change was induced by the binding of TnI–gold nanoparticle complexes to the antibodies at the test line. The silver-reducing reagents were subsequently released from the nanofibers via dissolution, which reduced silver ions to metallic silver around the gold nanoparticles and darkened the color of the test line. The hybrid nanofibers enhanced the detection limit of a commercial LFIA by up to 10 times when viewed with the naked eye while maintaining the assay time and convenience characteristics of this analysis.

我们开发了一种使用核-壳杂化纳米纤维来增强侧向流免疫测定（LFIA）灵敏度的简便方法，该纳米纤维在壳中包含水溶性聚合物，在核中包含还原银试剂。这些纳米纤维使用同轴电纺丝法生产，并直接沉积在测试线的硝酸纤维素（NC）膜上。当将掺有不同浓度的肌钙蛋白I（TnI）（一种心脏生物标志物）的人血清滴到吸收垫上时，TnI-金纳米颗粒复合物与测试线处的抗体结合会引起颜色变化。还原银的试剂随后通过溶解从纳米纤维中释放出来，这将银离子还原为金纳米颗粒周围的金属银，并使测试线的颜色变深。