This study reported a new, renewable and high-efficient strategy for anaerobic fermentation, i.e., using free ammonia (FA) to pretreat waste activated sludge (WAS) for 1 d and then combining with rhamnolipid (RL), by which the short-chain fatty acids (SCFA) production was remarkably improved. Experimental results showed the maximal SCFA production of 324.7 ± 13.9 mg COD/g VSS was achieved at 62.6 mg FA/L pretreatment combined with 0.04 g RL/g TSS, which was respectively 5.95-fold, 1.63-fold and 1.41-fold of that from control, FA pretreatment and RL pretreatment. Mechanism investigations revealed that FA + RL enhanced sludge solubilization and hydrolysis, providing more organics for subsequent SCFA production. It was also found that the combined method inhibited acidogenesis and methanogenesis, but the inhibition to methanogenesis was much severer than that to acidogenesis. Finally, the feasibility of NH₄⁺-N and PO³⁻₄-P, released in fermentation liquor, being recovered as magnesium ammonium phosphate (MAP) was confirmed.

这项研究报告了一种新的，可再生的，高效的厌氧发酵策略，即使用游离氨（FA）对废物活性污泥（WAS）进行预处理1 d，然后与鼠李糖脂（RL）结合，通过短链脂肪酸（SCFA）的产量显着提高。实验结果表明，在62.6 mg FA / L预处理与0.04 g RL / g TSS结合使用时，最大SCFA产量为324.7±13.9 mg COD / g VSS，分别是其的5.95倍，1.63倍和1.41倍来自对照，FA预处理和RL预处理。机理研究表明，FA + RL增强了污泥的溶解和水解，为随后的SCFA生产提供了更多的有机物。还发现该组合方法抑制了酸生成和甲烷生成，但是对产甲烷的抑制作用比对产酸的抑制作用要严重得多。最后，NH的可行性₄ ⁺ N和PO ^3- ₄ -P，在发酵液中释放时，回收作为磷酸镁铵（MAP）中得到证实。