Because of its attractive cost and yield, hierarchical assembly, in which constituent structures of lower hierarchy share a majority of components, is an appealing approach to scale up DNA self-assembly. A few strategies have already been investigated to combine preformed DNA nanostructures. In this study, we present a new hierarchical assembly method based on four-way toehold-mediated strand displacement to facilitate the combination of preformed DNA structural units. Employing such a method, we have constructed a series of higher-order structures composed of 5, 7, 9, 11, 13, and 15 preformed units respectively.

中文翻译：

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基于四路脚趾介导的链位移的DNA纳米结构的层次组装。

由于其诱人的成本和产量，分级组装是降低DNA自组装规模的一种有吸引力的方法，其中较低分级的组成结构共享大部分组件。已经研究了一些策略来组合预先形成的DNA纳米结构。在这项研究中，我们提出了一种新的基于四步脚趾介导的链置换的分层组装方法，以促进预先形成的DNA结构单元的组合。使用这种方法，我们构造了一系列分别由5、7、9、11、13和15个预制单元组成的高阶结构。