Di-butyl phthalate (DBP) is a widely used plasticizer, recalcitrant and hazardous organic compound with high detection frequencies and concentrations in water and soil that pose a great threat to human health. A novel endphytic bacterium strain N-1 capable of efficiently degrading DBP and utilizing it as sole carbon source was isolated from Ageratum conyzoides. This bacterium was identified as Bacillus subtilis based on its morphological characteristics and 16S rDNA sequence analysis. Under the optimal culture conditions (pH 7.0, 30 °C), degradation percentage of DBP (12.5–100 mg/L) was up to 95% within five days, and its biodegradation half-life was less than 7.23 h. Degradation percentage of high DBP concentration (200 mg/L) was relatively lower (89%) with half-life of 56.8 h. DBP was degraded by Bacillus subtilis N-1 into mono-butyl phthalate and phthalic acid as evidenced by GC-MS analysis. Bioaugmentation of Youngia japonica plant slurry with strain N-1 greatly accelerated DBP dissipation with 97.5% removal percentage (higher by 47% than non-inoculation). The results highlighted that strain N-1 has great potential for bioremediation by plant-endophyte partnerships and for lowering PAE accumulation in crops.

邻苯二甲酸二丁酯（DBP）是一种广泛使用的增塑剂，难降解性和有害有机化合物，在水和土壤中的检出频率和浓度较高，对人类健康构成极大威胁。从香叶A香中分离出一种新型的能有效降解DBP并将其作为唯一碳源的内生细菌菌株N-1 。该细菌被鉴定为枯草芽孢杆菌根据其形态特征和16S rDNA序列分析。在最佳培养条件下（pH 7.0，30°C），DBP的降解百分率（12.5-100 mg / L）在五天内可达到95％，其生物降解半衰期小于7.23 h。高DBP浓度（200 mg / L）的降解率相对较低（89％），半衰期为56.8 h。GC-MS分析表明，枯草芽孢杆菌N-1将DBP降解为邻苯二甲酸单丁酯和邻苯二甲酸。粳稻的生物强化菌株N-1的植物泥浆极大地促进了DBP的消散，去除率达97.5％（比未接种高47％）。结果表明，N-1菌株具有巨大的潜力，可通过植物与内生植物的伙伴关系进行生物修复，并降低农作物中PAE的积累。