Abstract Although clove oil is widely used in food preservation, very little is known about its possible antibacterial mechanism. In order to reveal its antibacterial mechanism, the inhibitory effect of clove oil on Listeria monocytogenes and its effect on cell membrane, respiratory metabolism, and its interaction with DNA were investigated, respectively. The results showed the treatment of clove oil can cause the leakage of three biological macromolecules (protein, ATP and DNA) and the reduction of two intracellular enzymes (β-galactosidase and AKP) activities, indicating that clove oil has an effect on cell membrane permeability. The inhibition rate and superposition rate test confirmed that the pathway of clove oil affecting the respiratory metabolism of Listeria monocytogenes is TCA pathway. The treatment of clove oil can reduce the activity of three key enzymes (isocitrate dehydrogenase, citrate synthase and α-ketoglutarate dehydrogenase) in TCA pathway, and affect the content of metabolites in the pathway. The results of ultraviolet absorption spectroscopy showed that eugenol, the main component of clove oil, can change the structure of DNA via the formation of eugenol-DNA chimera.

中文翻译：

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丁香油对单核细胞增生李斯特菌的抑菌机制

摘要 尽管丁香油广泛用于食品保鲜，但对其可能的抗菌机制知之甚少。为了揭示其抗菌机制，分别研究了丁香油对单核细胞增生李斯特菌的抑制作用及其对细胞膜、呼吸代谢和与DNA相互作用的影响。结果表明丁香油处理可引起三种生物大分子（蛋白质、ATP和DNA）的渗漏和两种细胞内酶（β-半乳糖苷酶和AKP）活性的降低，说明丁香油对细胞膜通透性有影响. 抑制率和叠加率试验证实丁香油影响单核细胞增生李斯特菌呼吸代谢的途径为TCA途径。丁香油处理可降低TCA途径中三种关键酶（异柠檬酸脱氢酶、柠檬酸合酶和α-酮戊二酸脱氢酶）的活性，影响该途径中代谢物的含量。紫外吸收光谱结果表明丁香油的主要成分丁香酚可以通过形成丁香酚-DNA嵌合体来改变DNA的结构。