The cobalt incorporated mesoporous silica nanomaterials (Co-MSN) with different ratios of Si/Co in the framework were synthesized. The as-obtained products were characterized by X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy (FT-IR), X-ray fluorescence (XRF), field emission scanning electron microscopy (FESEM), transmission electron microscope (TEM), N₂ adsorption/desorption (BET) and UV–Vis spectroscopic techniques. The effect of the various content of Co incorporated into the framework on the catalytic activity was studied. The Co-MSN samples exhibited an excellent catalytic activity toward the reduction of hydrogen peroxide (H₂O₂) and the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate and followed Michaelis−Menten kinetics. It means that they have intrinsic peroxidase-like activity. The results showed that the Co-MSN catalyst with Si/Co = 75 (as the optimum ratio), has the higher affinity to ABTS and H₂O₂ and higher catalytic efficiency. These features made this nanocatalyst suitable to apply for the determination of H₂O₂ and glucose. The linear range for detecting H₂O₂ was from 22.5 μM to 0.2 mM with a detection limit of 6.7 μM. Coupled with glucose oxidase, the Co-MSN(75) was successfully used for the determination of glucose with the linear range of 11.6 μM−0.1 mM and a detection limit of 3.5 μM.

合成了骨架中具有不同Si / Co比的掺钴介孔二氧化硅纳米材料（Co-MSN）。通过X射线衍射分析（XRD），傅立叶变换红外光谱（FT-IR），X射线荧光（XRF），场发射扫描电子显微镜（FESEM），透射电子显微镜（TEM）对所得产物进行表征。 ），N ₂吸附/解吸（BET）和UV-Vis光谱技术。研究了掺入骨架中的各种含量的Co对催化活性的影响。Co-MSN样品对还原过氧化氢（H ₂ O ₂）和2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）的氧化作为生色底物，并遵循Michaelis-Menten动力学。这意味着它们具有固有的过氧化物酶样活性。结果表明，Si / Co = 75（最佳比例）的Co-MSN催化剂对ABTS和H ₂ O _2的亲和力更高，催化效率更高。这些特征使得该纳米催化剂适用于测定H ₂ O ₂和葡萄糖。检测H ₂ O ₂的线性范围的检测限为22.5μM至0.2 mM，检测极限为6.7μM。结合葡萄糖氧化酶，Co-MSN（75）成功用于线性范围为11.6μM-0.1mM和检测限为3.5μM的葡萄糖的测定。