Foodborne pathogen is the primary cause of foodborne disease outbreak. Given its great damage, a sensitive, simple and rapid detection method is demanded. Herein, we described a self-priming polydimethylsiloxane (PDMS)/paper hybrid microfluidic chip, termed SPH chip, with mixed-dye-loaded loop-mediated isothermal amplification (LAMP) for multiplex foodborne pathogens detection. Staphylococcus aureus (SA) and Vibrio parahaemolyticus (VP) were chosen to verify the novel method. Compared to other similar detection devices, the SPH chip required easier fabrication process, less operation steps and lower cost. Additionally, the reaction result, especially for the weak-positive reaction, could be judged more accurately and conveniently due to the use of mixed-dye. Without pre-enrichment of bacteria in the food contaminated sample, the limit of detection (LOD) reached down to 1000 CFU mL-1 with high specific. Additionally, for fully exploiting the potential of SPH chip, a conceptual eight-channel detection chip was also developed. Overall, the reliable and excellent result demonstrated that the novel method had great potential to be applied in the wider range of pathogens detection or disease diagnose, especially in some resource-limited area.

中文翻译：

---

使用混合染料加载环介导的等温扩增试验开发用于多重食源性病原体检测的自吸 PDMS/纸混合微流控芯片

食源性病原体是食源性疾病爆发的主要原因。鉴于其巨大的危害，需要一种灵敏、简单、快速的检测方法。在此，我们描述了一种自吸聚二甲基硅氧烷 (PDMS)/纸混合微流控芯片，称为 SPH 芯片，具有用于多重食源性病原体检测的混合染料负载环介导等温扩增 (LAMP)。选择金黄色葡萄球菌 (SA) 和副溶血性弧菌 (VP) 来验证新方法。与其他类似的检测设备相比，SPH芯片需要更简单的制造工艺、更少的操作步骤和更低的成本。此外，由于混合染料的使用，可以更准确、方便地判断反应结果，尤其是弱阳性反应。未经食品污染样品中细菌的预富集，检测限 (LOD) 低至 1000 CFU mL-1，具有高特异性。此外，为了充分发挥SPH芯片的潜力，还开发了概念性的八通道检测芯片。总体而言，可靠且优异的结果表明，该新方法具有巨大的潜力，可应用于更广泛的病原体检测或疾病诊断，尤其是在一些资源有限的地区。