当前位置: X-MOL 学术Biomater. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Electrically conductive MEH-PPV:PCL electrospun nanofibres for electrical stimulation of rat PC12 pheochromocytoma cells†
Biomaterials Science ( IF 6.6 ) Pub Date : 2018-07-09 00:00:00 , DOI: 10.1039/c8bm00559a
Rajiv Borah 1, 2, 3, 4, 5 , Ganesh C. Ingavle 6, 7, 8, 9, 10 , Susan R. Sandeman 6, 7, 8, 9, 10 , Ashok Kumar 1, 2, 3, 4, 5 , Sergey Mikhalovsky 6, 7, 8, 9, 10
Affiliation  

The purpose of this study was to prepare an electrically conducting poly[2-methoxy-5-(2-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV) based nanofibrous scaffold and to investigate the synergetic effect of nanofibre structure and electrical stimulation on neuronal growth for possible use in nerve repair. Nanofibres were produced by electrospinning of blended MEH-PPV with polycaprolactone (PCL) at a ratio of 20 : 80, 40 : 60, 50 : 50 and 60 : 40 (v/v). A better electrical conductivity was achieved by using core-sheath structured nanofibres of PCL (core) and MEH-PPV (sheath) produced using the coaxial electrospinning technique. The highest electrical conductivity was observed in the core-sheath nanofibres, while it increased with increasing concentration of MEH-PPV for the blended electrospun nanofibres. The biocompatibility of the electrospun nanofibres was confirmed by MTS and live-dead staining assays using 3T3 fibroblasts and a neuronal rat pheochromocytoma (PC12) cell line. Beta (III) tubulin immunochemistry showed that PC12 cells differentiated into sympathetic neurons on these porous and stiffer electrospun nanofibres coated with collagen I. Improved cell morphology and attachment on the collagen I coated electrospun meshes has been confirmed by SEM analysis. Significant enhancement in neurite formation and neurite outgrowth of PC12 cells on the conductive scaffolds under electrical potential of 500 mV cm−1 for 2 h day−1 suggests the potential use of these scaffolds for nerve repair.

中文翻译:

导电的MEH-PPV:PCL电纺纳米纤维可电刺激大鼠PC12嗜铬细胞瘤细胞

这项研究的目的是制备一种导电的聚[2-甲氧基-5-(2-乙基-己氧基)-1,4-亚苯基亚乙烯基](MEH-PPV)基纳米纤维支架,并研究纳米纤维的协同作用。对神经元生长的结构和电刺激,可能用于神经修复。通过以20:80、40:60、50:50和60:40(v / v)的比例将MEH-PPV与聚己内酯(PCL)进行静电纺丝生产纳米纤维。通过使用使用同轴电纺丝技术生产的PCL(芯)和MEH-PPV(鞘)的芯鞘结构纳米纤维,可以获得更好的导电性。在芯鞘纳米纤维中观察到最高的电导率,而对于混合电纺纳米纤维,它随着MEH-PPV浓度的增加而增加。使用3T3成纤维细胞和神经元大鼠嗜铬细胞瘤(PC12)细胞系通过MTS和活死染色测定法证实了电纺纳米纤维的生物相容性。Beta(III)微管蛋白免疫化学表明,在这些涂有胶原蛋白I的多孔且较硬的电纺纳米纤维上,PC12细胞分化为交感神经元。通过SEM分析已证实,细胞形态和附着在涂有胶原蛋白I的电纺网片上的细胞形态得到改善。在500 mV cm的电势下,导电支架上PC12细胞的神经突形成和神经突向外生长显着增强 Beta(III)微管蛋白免疫化学表明,在这些涂有胶原蛋白I的多孔且较硬的电纺纳米纤维上,PC12细胞分化为交感神经元。通过SEM分析已确认细胞形态和附着在胶原I涂层的电纺网上的附着力得到改善。在500 mV cm的电势下,导电支架上PC12细胞的神经突形成和神经突向外生长显着增强 Beta(III)微管蛋白免疫化学表明,在这些涂有胶原蛋白I的多孔且较硬的电纺纳米纤维上,PC12细胞分化为交感神经元。通过SEM分析已证实,细胞形态和附着在涂有胶原蛋白I的电纺网片上的细胞形态得到改善。在500 mV cm的电势下,导电支架上PC12细胞的神经突形成和神经突向外生长显着增强-1持续2小时,第-1天表明这些支架可潜在地用于神经修复。
更新日期:2018-07-09
down
wechat
bug