Two novel cyclopeptides with special skeleton, namely, dolyemycins A (1) and B (2) were isolated from Streptomyces griseus subsp. griseus HYS31 by bio-guided isolation. Their structures were elucidated by detailed analysis of spectroscopic data. These two compounds were cyclopeptides containing eleven amino acids including five unusual amino acids (hydroxyglycine, 3-hydroxyleucine, 3-phenylserine, β-hydroxy-O-methyltyrosine, 2,3-diaminobutyric acid) in both of them and an extra nonprotein amino acids (3-methylaspartic acid) in Dolyemycin B only. Dolyemycins A and B performed antiproliferative activity against human lung cancer A549 cells with IC₅₀ values of 1.0 and 1.2 µM, respectively.

中文翻译：

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Dolyemycins A和B，两个从灰链霉菌亚种分离的新型环肽。Griseus HYS31。

从灰链霉菌亚种中分离出两个具有特殊骨架的新型环肽，即多柳霉素A（1）和B（2）。griseus HYS31通过生物引导分离。通过详细分析光谱数据阐明了它们的结构。这两种化合物是含有11个氨基酸的环肽，其中包括5个不同的氨基酸（羟基甘氨酸，3-羟基亮氨酸，3-苯基丝氨酸，β-羟基-O-甲基酪氨酸，2,3-二氨基丁酸）和一个额外的非蛋白质氨基酸（3-甲基天冬氨酸）仅在多霉素B中使用。多柳霉素A和B对人肺癌A549细胞具有抗增殖活性，IC ₅₀值分别为1.0和1.2 µM。