Type 1 diabetes is an autoimmune disease leading to insulin deficiency. Autoantibodies to beta cell proteins are already present in the asymptomatic phase of type 1 diabetes. Recent findings have suggested a number of additional minor autoantigens in patients with type 1 diabetes. We have established luciferase immunoprecipitation systems (LIPS) for anti-MTIF3, anti-PPIL2, anti-NUP50 and anti-MLH1 and analyzed samples from 500 patients with type 1 diabetes at onset of clinical disease and 200 healthy individuals who had a family history of type 1 diabetes but no evidence of beta cell autoantibodies. We show significantly higher frequencies of anti-MTIF3, anti-PPIL2 and anti-MLH1 in recent onset type 1 diabetes patients in comparison to controls. In addition, antibodies to NUP50 were associated with HLA-DRB1*03 and antibodies to MLH1 were associated with HLA-DRB1*04 genotypes.

1型糖尿病是导致胰岛素缺乏的自身免疫性疾病。β细胞蛋白的自身抗体已经存在于1型糖尿病的无症状阶段。最近的发现提示1型糖尿病患者还有许多其他次要自身抗原。我们已经建立了针对抗MTIF3，抗PPIL2，抗NUP50和抗MLH1的荧光素酶免疫沉淀系统（LIPS），并分析了500例临床疾病发作时的1型糖尿病患者和200例具有家族史的健康个体的样本1型糖尿病，但没有β细胞自身抗体的证据。与对照组相比，我们在近期发病的1型糖尿病患者中显示出更高的抗MTIF3，抗PPIL2和抗MLH1频率。此外，