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Development of immunosorbents for the analysis of forchlorfenuron in fruit juices by ion mobility spectrometry
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-07-07 , DOI: 10.1007/s00216-018-1213-5
Alejandro Orellana-Silla , Sergio Armenta , Miguel de la Guardia , Josep V. Mercader , Francesc A. Esteve-Turrillas

The advantages of using smart materials as immunosorbents in the analysis of complex matrices by ion mobility spectrometry (IMS) have been highlighted in this study. A novel analytical method has been proposed for the sensitive, selective, and fast determination of residues of the plant growth regulator forchlorfenuron in fruit juices. Three different monoclonal antibodies (s3#22, p2#21, and p6#41) were employed for the production of immunosorbents, based on Sepharose gel beads, which were characterized in terms of loading capacity, solvent resistance, and repeatability for its use in solid-phase extraction (SPE). Immunosorbents that were prepared with antibody p6#44 provided the best performance, with a loading capacity of 0.97 μg, a 10% (v/v) 2-propanol tolerance, and a reusability of at least eight uses. The SPE procedure involved the use of a column with 0.15 g Sepharose beads, containing 0.5 mg antibody, which was loaded to 20 mL of the sample, washed with 2 mL of water plus 2 mL of 10% (v/v) 2-propanol, and eluted with 2 mL of 2-propanol. The cleaned extract was directly analyzed by IMS, giving a limit of detection of 2 μg L−1 with a relative standard deviation of 7.6%. Trueness was assessed by the analysis of blank grape and kiwifruit juice samples spiked with forchlorfenuron concentrations from 10 to 400 μg L−1, with recoveries from 80 to 115%. The analytical performance of the proposed immunosorbent was compared with conventional extraction and cleanup methods, such as QuEChERS and C18-based SPE, giving the cleanest extracts for accurate determinations of forchlorfenuron by IMS.

中文翻译:

离子淌度光谱法分析果汁中甲草胺的免疫吸附剂的开发

这项研究突出了使用智能材料作为免疫吸附剂通过离子迁移谱(IMS)分析复杂基质的优势。提出了一种新颖的分析方法,用于敏感,选择性和快速测定果汁中敌百虫的植物生长调节剂的残留量。基于琼脂糖凝胶珠,使用了三种不同的单克隆抗体(s3#22,p2#21和p6#41)来生产免疫吸附剂,其负载量,耐溶剂性和重复使用性均得到了表征。固相萃取(SPE)。用抗体p6#44制备的免疫吸附剂性能最佳,负载量为0.97μg,10%(v / v)对2-丙醇的耐受性,至少可重复使用八次。SPE程序涉及使用装有0.15 g Sepharose珠的色谱柱,其中含有0.5 mg抗体,将其上样至20 mL样品,用2 mL水和2 mL 10%(v / v)2-丙醇洗涤,并用2 mL 2-丙醇洗脱。清洁的提取物直接通过IMS分析,检出限为2μgL -1,相对标准偏差为7.6%。通过分析空白葡萄和猕猴桃汁样品中的甲虫草隆浓度为10至400μgL -1的情况来评估真实性,回收率从80到115%。将拟议的免疫吸附剂的分析性能与常规提取和纯化方法(例如QuEChERS和基于C 18的SPE)进行了比较,从而获得了最清洁的提取物,可通过IMS准确测定甲草胺。
更新日期:2018-07-08
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