Genotoxicity studies of plants with medicinal and nutritional properties are recommended by international regulatory agencies as part of the risk assessment. Due to their consumption as food, nutraceutical use and ethnopharmacological relevance, Campomanesia pubescens represents one of these plants to be studied. The aim of the present study was to evaluate the genotoxic, cytotoxic potential and clathogenic effects of the ethanolic extract obtained from the pulp of C. pubescens (EEFCP) fruits on rats submitted to experimental genotoxicity models and through the SMART test performed in Drosophila melanogaster. The comet assay and the micronucleus test were performed on peripheral and bone marrow blood, respectively, of Wistar rats orally treated with EEFCP at doses of 125, 250, 500 and 1000 mg per kg per bw for 28 days. In the SMART test, the standard cross between three mutant D. melanogaster strains was used. Larvae were treated with EEFCP at different concentrations and the wings of adult flies were evaluated for the presence/frequency of mutant spots and compared to the negative control group. Phytochemical analysis of EEFCP indicated high levels of flavonoids. The tests performed in rats showed that EEFCP did not present significant genotoxic or clastogenic effects. The biotransformation metabolites of EEFCP did not present genotoxic activity, as demonstrated by the SMART test. Together, all results indicate that, under the experimental conditions used, EEFCP did not reveal any preclinical genetic toxicity. Therefore, the safe consumption can be fomented increasing, consequently, the economic liquidity in the industrial market from the fruits of guavira.

中文翻译：

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毛果樟脑油（Mart。ex DC。）O.BERG（番石榴）果实中 乙醇提取物的临床前安全性评估：遗传毒性和杀乳作用的分析†

作为风险评估的一部分，国际监管机构建议对具有药用和营养性质的植物进行基因毒性研究。由于它们作为食物消费，营养保健用途和民族药理学意义，因此，樟脑草代表了这些要研究的植物之一。本研究的目的是评估基因毒性，细胞毒性潜力，并从纸浆中获得的乙醇提取物的效果clathogenic C.毛竹（EEFCP）水果提交给实验模型的基因毒性和通过SMART测试大鼠进行果蝇。分别对经EEFCP口服处理的Wistar大鼠以125、250、500和1000 mg / kg / bw的剂量进行28天的彗星试验和微核试验。在SMART测试中，三个突变体D. melanogaster之间的标准杂交使用了菌株。用不同浓度的EEFCP处理幼虫，评估成年苍蝇翅膀上突变点的存在/频率，并与阴性对照组进行比较。EEFCP的植物化学分析表明，类黄酮含量很高。在大鼠中进行的测试表明，EECFP并未表现出明显的遗传毒性或致裂作用。如SMART试验所示，EEFCP的生物转化代谢物没有遗传毒性。总之，所有结果表明，在使用的实验条件下，EECFP并未显示出任何临床前遗传毒性。因此，可以促进安全消费，从而从番石榴果实中增加工业市场中的经济流动性。