Telomerase is considered as a widely accepted cancer biomarker for early cancer diagnostics. Herein, we develop a simple, ultrahigh sensitivity method for detection of telomerase activity, which relied on that RecA-GFP fusion proteins wrapped around telomeric DNA to form fluorescence bouquets. RecA-GFP fusion protein was synthesized through fusion protein technology. In the presence of telomerase, telomerase elongation products are wrapped around by RecA-GFP fusion protein to form big fluorescent bouquets, which resulted in strong fluorescence. This method has the linear range from 50 to 1000 HeLa cells and the detection limit is 8 HeLa cells, based on a signal-to-noise ratio (S/N) of 3. Compared with conventional methods, this method has the advantages of low toxicity, outstanding sensitivity, and excellent selectivity. Hence, it provides a promising approach for the detection of telomerase activity and diagnosis of cancer.

中文翻译：

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一种使用融合蛋白束检测端粒酶活性的简单而灵敏的荧光方法

端粒酶被认为是用于早期癌症诊断的广泛接受的癌症生物标志物。在此，我们开发了一种简单、超高灵敏度的端粒酶活性检测方法，该方法依赖于包裹在端粒 DNA 周围的 RecA-GFP 融合蛋白形成荧光花束。RecA-GFP融合蛋白是通过融合蛋白技术合成的。在端粒酶存在下，端粒酶延伸产物被RecA-GFP融合蛋白包裹，形成大的荧光花束，产生强烈的荧光。该方法的线性范围为 50 到 1000 个 HeLa 细胞，检出限为 8 个 HeLa 细胞，基于信噪比 (S/N) 为 3。与传统方法相比，该方法具有低毒性、出色的灵敏度和出色的选择性。因此，