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Persistence and spatial variation of antibiotic resistance genes and bacterial populations change in reared shrimp in South China
Environment International ( IF 11.8 ) Pub Date : 2018-07-07 , DOI: 10.1016/j.envint.2018.07.007
Haochang Su , Xiaojuan Hu , Yu Xu , Wujie Xu , Xiaoshuai Huang , Guoliang Wen , Keng Yang , Zhuojia Li , Yucheng Cao

More attention has been paid to the abundance and diversity of antibiotic resistance genes (ARGs) in aquatic environments. However, few studies have investigated the persistence and spatial variation of ARGs in aquatic organisms. This study investigated the occurrence and abundance of ARGs and the bacterial populations in shrimp intestinal tracts during the rearing period in different regions of Guangdong, South China. The results showed that sul1, sul2, qnrD, and floR were the predominant ARGs. Compared with those of juvenile shrimp, the total concentrations of ARGs in the intestinal tract of adult shrimp in three shrimp farms were 2.45–3.92 times higher (p < 0.05), and the bacterial populations in the adult shrimp intestinal tract changed considerably. Redundancy analysis (RDA) showed that the abundance of Proteobacteria, Firmicutes, and Verrucomicrobia in Farms A, B, and C, respectively, were strongly positively correlated with the most abundant and predominant genes (sul1 and qnrD for Farm A; floR and sul2 for Farm B; floR and sul2 for Farm C) in the shrimp intestinal tract. The results of this study indicated that ARGs gained persistence in the developmental stages of the reared shrimp. Different phyla of predominant bacteria were responsible for the increase of ARGs abundance in the shrimp intestinal tract in different regions. This study represents a case study of the persistence and spatial variation of ARGs in aquaculture and can be a reference for the determination of harmful impacts of ARGs on food safety and human health.



中文翻译:

华南对虾抗生素抗性基因和细菌种群的持久性和空间变异

水生环境中抗生素耐药性基因(ARG)的丰富性和多样性已引起更多关注。然而,很少有研究调查水生生物中ARG的持久性和空间变化。本研究调查了华南广东不同地区养殖期间虾虾肠道中ARGs的发生和数量以及细菌种群。结果显示, sul 1, sul 2, qnr D和flo R是主要的ARG。与未成年虾相比,三个虾场中成年虾肠道中ARGs的总浓度高2.45-3.92倍( p <0.05),并且成年虾肠道中的细菌种群发生了很大变化。冗余分析(RDA)显示,农场A,B和C的细菌总数,菌毛和微生物菌群的丰度分别与最丰富和主要的基因(农场A的sul 1和qnr D;flo R和B农场的sul 2;flo R和sul2在农场C)虾的肠道中。这项研究的结果表明,ARGs在养殖虾的发育阶段获得了持久性。不同区域的主要细菌菌群不同,导致虾肠道ARGs的丰度增加。这项研究是对水产养殖中ARGs的持久性和空间变化的案例研究,可以为确定ARGs对食品安全和人类健康的有害影响提供参考

更新日期:2018-07-12
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