Abstract Herein, we prepared a series of novel hydroxyapatite (HA) nanowire and gelatin composite films with varying HA to gelatin ratios, attempting to simulate the major features of the natural extracellular matrix of bone. The results showed that the ratio of HA nanowires to gelatin had a great influence on the physicochemical and biological properties of the composites. The morphology of the HA/gelatin 7/3 composite film had a fibrous and porous architecture, while the 5/5 and 3/7 samples led to the formation of non-porous rough surfaces. Additionally, an increase in HA content enhanced the composite mechanical properties, with a maximum tensile strength of 114 ± 8 MPa, respectively. The HA/gelatin composite films exhibited good cell adhesion and proliferation. Osteogenic differentiation of mouse bone marrow mesenchymal stem cells on the 7/3 composite film was superior to that of the remaining films, as evident by alkaline phosphatase activity, calcium deposition, and gene expression. Thus, the HA/gelatin 7/3 composite film prepared herein has potential as a bone substitute for bone repair and regeneration.

中文翻译：

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用于骨组织再生的仿生羟基磷灰石/明胶复合材料：体外制造、表征和成骨分化

摘要 在此，我们制备了一系列具有不同 HA 与明胶比例的新型羟基磷灰石 (HA) 纳米线和明胶复合膜，试图模拟骨的天然细胞外基质的主要特征。结果表明，HA纳米线与明胶的比例对复合材料的理化和生物学性能有很大影响。HA/明胶 7/3 复合膜的形态具有纤维状和多孔结构，而 5/5 和 3/7 样品导致形成无孔粗糙表面。此外，HA 含量的增加增强了复合材料的机械性能，最大拉伸强度分别为 114 ± 8 MPa。HA/明胶复合膜表现出良好的细胞粘附和增殖。7/3 复合膜上小鼠骨髓间充质干细胞的成骨分化优于其余膜，如碱性磷酸酶活性、钙沉积和基因表达所证明的。因此，本文制备的HA/明胶7/3复合膜具有作为骨修复和再生的骨替代物的潜力。