It is of great significance to probe the effect of fungicides on living fungal cell using fluorescent sensors, which can provide visual evidence for their fungicidal mechanism. In this work, the effect of thiram (0.0–80.0 µM) on the membrane of fungal cell was investigated by monitoring the influxes of amine terminated carbon dots (CDs–NH₂), carboxylate group coated carbon dots (CDs–COO-) and Cu²⁺ into cells via fluorescent imaging. Results showed that the internalizations of the positively charged CDs–NH₂ and Cu²⁺ into fungal cells were promoted by thiram at the concentration from 0.0 to 60.0 (40.0 for Cu²⁺) µM, but their internalizations decreased with a further increase in thiram concentration from 60.0 (40.0 for Cu²⁺) to 80.0 µM. On the other hand, the internalization of the passively charged CDs–COO- was not affected by thiram. Therefore, it can be deduced that the effect of thiram on the membrane of fungal cell probed by the fluorescent carbon dots is stimulating the non-selective cation channel up to a certain point in a dose dependent manner. Additionally, an approach was built for the detection of thiram with high sensitivity and selectivity in the presence of excessive Cu²⁺.

利用荧光传感器探查杀真菌剂对活菌细胞的作用具有重要意义，可以为杀真菌剂的杀真菌机理提供直观的证据。在这项工作中，通过监测胺封端的碳点（CDs–NH ₂），羧化基团涂覆的碳点（CDs–COO-）和硫的流入量，研究了硫醇（0.0–80.0 µM）对真菌细胞膜的影响。Cu ²⁺通过荧光成像进入细胞。结果表明，带正电的CD-NH的internalizations ₂和Cu ²⁺进入真菌细胞通过福美双在浓度促进从0.0到60.0（40.0对Cu ²⁺）µM，但它们的内在化随着硫胺浓度的进一步提高而从60.0（Cu ²⁺为40.0 ）增加到80.0 µM。另一方面，被动充电的CD-COO-的内在化不受锡兰姆的影响。因此，可以推断出，硫胺素对由荧光碳点探测的真菌细胞膜的作用以剂量依赖性方式将非选择性阳离子通道刺激至特定点。此外，建立了一种在过量Cu ²⁺的情况下以高灵敏度和选择性检测噻喃的方法。