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Integrated microfluidic paper-based system for determination of whole blood albumin
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2018-07-07 , DOI: 10.1016/j.snb.2018.07.010
Ruey-Jen Yang , Chin-Chung Tseng , Wei-Jhong Ju , Lung-Ming Fu , Meng-Ping Syu

A method is proposed for determining the concentration of albumin (ALB) in human whole blood samples using a 3-dimensional (3D) microfluidic paper-based chip and a smart detection module. In fabricating the paper-based chip, the reaction area is implanted with bromocresol green (BCG) reagent and the device is stored in a nitrogen (N2) environment at a temperature of —15 °C until needed for use. In the detection process, 10 μl of whole blood is dropped onto the entrance area of the chip and the plasma within the sample diffuses through a separation channel into the reaction zone. A reaction is induced between the ALB in the plasma and the BCG reagent in the reaction area by heating the chip at 37 °C for 6 min. The ALB concentration is then detected using a colorimetry technique implemented on a cell phone in the form of a self-written app. It is shown that the detection results obtained for 40 pure ALB samples and 30 whole blood samples are consistent with those obtained using a traditional spectrophotometry method (R2 = 0.9837 and R2 = 0.9968). The proposed detection system thus provides a reliable tool for practical ALB determination purposes.



中文翻译:

集成的基于微流纸的全血白蛋白测定系统

提出了一种使用3D(3D)微流控纸基芯片和智能检测模块确定人全血样品中白蛋白(ALB)浓度的方法。在制造纸基芯片时,在反应区域中注入溴甲酚绿(BCG)试剂,并将该设备存储在氮气(N 2)环境中的温度为-15°C,直到需要使用为止。在检测过程中,将10μl全血滴到芯片的入口区域,样品中的血浆通过分离通道扩散到反应区中。通过将芯片在37°C下加热6分钟,可以在血浆中的ALB和反应区域中的BCG试剂之间引发反应。然后使用在手机上以自写应用程序形式实现的比色技术检测ALB浓度。结果表明,对40个纯ALB样品和30个全血样品的检测结果与使用传统分光光度法获得的检测结果一致(R 2  = 0.9837和R 2 = 0.9968)。因此,所提出的检测系统为实际的ALB确定目的提供了可靠的工具。

更新日期:2018-07-07
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