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Comprehensive overview and recent advances in proteomics MS based methods for food allergens analysis
Trends in Analytical Chemistry ( IF 13.1 ) Pub Date : 2018-07-05 , DOI: 10.1016/j.trac.2018.06.016
Linda Monaci , Elisabetta De Angelis , Nicola Montemurro , Rosa Pilolli

Food allergies are a serious health concern with increasing worldwide prevalence. Food legislation in place in several countries, requires detailed declaration of allergens in foods implying capability of methodologies to reliably trace food allergens. However, detecting and quantifying food allergens remains a challenge. Current common methods for food allergen analysis utilize antibody-based assays although some drawbacks are encountered such as matrix/processing effects and epitope masking especially when dealing with complex and processed foods. Therefore, sensitive, reliable, robust, fast, reproducible, and standardized methods are necessary for improved allergen analysis and reduce the risk of allergen contamination. In the last decade, mass spectrometry (MS) techniques have been developed and applied with success to food allergen detection. This review compares different aspects of food allergen quantification using advanced MS techniques including multiple reaction monitoring. The latter provides low limits of quantification for multiple allergens in complex food matrices, while being robust and reproducible.



中文翻译:

基于蛋白质组学MS的食品过敏原分析方法的全面概述和最新进展

随着世界范围的流行,食物过敏是严重的健康问题。多个国家/地区已制定食品法规,要求详细声明过敏原在食品中的应用暗示了方法学能够可靠地追踪食品过敏原的能力。然而,检测和量化食物过敏原仍然是一个挑战。尽管遇到一些缺点,例如基质/加工效果和表位掩盖,特别是在处理复杂和加工食品时,当前用于食品过敏原分析的常用方法仍采用基于抗体的分析方法。因此,必须采用灵敏,可靠,可靠,快速,可重现和标准化的方法来改善过敏原分析并降低过敏原污染的风险。在过去的十年中,质谱(MS)技术得到了发展,并成功应用于食品过敏原的检测。这篇综述比较了使用先进的MS技术(包括多反应监测)对食物过敏原定量的不同方面。

更新日期:2018-07-12
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