Abstract Amperometric biosensors based on alcohol dehydrogenase (ADH) and alcohol oxidase (AOx) were developed for analysis of ethanol, methanol and their mixtures. The first biosensor based on ADH responds only to ethanol, the second biosensor based on AOx responds to both methanol and ethanol. Screen printed carbon electrodes (SPCEs) were used as the base electrodes. They were first modified with nanoparticles (multiwalled carbon nanotubes and gold nanoparticles) and polyneutral red film. Then the enzyme ADH was immobilized on them to prepare ADH based biosensor for ethanol analysis or the enzyme AOx was immobilized on them to prepare AOx based biosensor for methanol analysis. The analytical characterization parameters sensitivity, linear range, limits of detection and quantification were found as 0.432 μA·mM−1, 178.5–1000 μM, 53.5 μM and 178.5 μM, respectively, for ethanol analysis with AOx based biosensor, as 0.509 μA·mM−1, 335.9–1000 μM, 100.8 μM and 335.9 μM, respectively, for methanol analysis with AOx based biosensor and 0.490 μA·mM−1, 320–1000 μM, 96.1 μM and 320.2 μM, respectively for ethanol analysis with ADH based biosensor. The same parameters were also determined for the analysis of different mixtures with AOx based biosensor. The developed biosensors have been tested on a commercial alcoholic drink for the analysis of its ethanol and methanol contents.

中文翻译：

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使用导电聚合物和纳米材料修饰的丝网印刷碳电极开发电流生物传感器，用于分析乙醇、甲醇及其混合物

摘要 开发了基于乙醇脱氢酶 (ADH) 和乙醇氧化酶 (AOx) 的电流型生物传感器，用于分析乙醇、甲醇及其混合物。第一个基于 ADH 的生物传感器仅对乙醇有响应，基于 AOx 的第二个生物传感器对甲醇和乙醇都有响应。丝网印刷碳电极 (SPCE) 用作基础电极。它们首先用纳米粒子（多壁碳纳米管和金纳米粒子）和多中性红膜进行了修饰。然后将酶ADH固定在它们上以制备用于乙醇分析的基于ADH的生物传感器或将酶AOx固定在其上以制备用于甲醇分析的基于AOx的生物传感器。分析表征参数灵敏度、线性范围、检测限和定量限为 0.432 μA·mM-1、178.5–1000 μM、53.5 μM 和 178。分别为 5 μM，用于使用基于 AOx 的生物传感器的乙醇分析，分别为 0.509 μA·mM-1、335.9–1000 μM、100.8 μM 和 335.9 μM，用于使用基于 AOx 的生物传感器和 0.490 μA·mM-1、320 的甲醇分析–1000 μM、96.1 μM 和 320.2 μM，分别用于使用基于 ADH 的生物传感器进行乙醇分析。使用基于 AOx 的生物传感器分析不同混合物时也确定了相同的参数。开发的生物传感器已经在商业酒精饮料上进行了测试，以分析其乙醇和甲醇含量。使用基于 AOx 的生物传感器分析不同混合物时也确定了相同的参数。开发的生物传感器已经在商业酒精饮料上进行了测试，以分析其乙醇和甲醇含量。使用基于 AOx 的生物传感器分析不同混合物时也确定了相同的参数。开发的生物传感器已经在商业酒精饮料上进行了测试，以分析其乙醇和甲醇含量。