当前位置: X-MOL 学术Microbiol. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
An essential role for hfq involved in biofilm formation and virulence in serotype 4b Listeria monocytogenes
Microbiological Research ( IF 6.7 ) Pub Date : 2018-07-07 , DOI: 10.1016/j.micres.2018.07.001
Hao Yao , Meiqin Kang , Yuting Wang , Youwei Feng , Suwei Kong , Xuexue Cai , Zhiting Ling , Sisi Chen , Xin’an Jiao , Yuelan Yin

Regulator factor Hfq has been widely detected among both Gram-positive and Gram-negative bacteria; however, its role in Gram-positive bacteria is less well established and varies among species. In Listeria monocytogenes (Lm), an organism able to adapt to a range of environments and live both saprobiotic and parasitic lifestyles, the role of Hfq is not fully understood. Serotype 4b Listeria monocytogenes strains associated with the majority of listeriosis outbreak, while the function of hfq in serotype 4b strains still not referenced. Here, we constructed hfq deletion and reversion mutants of serotype 4b Lm NTSN and analysed the biological characteristics both in vitro and in vivo. The deletion of hfq resulted in a growth deficiency in medium containing 4.5% ethanol or 1% Triton X-100, and the growth of the mutant was significantly reduced at 4 °C. Furthermore, the hfq deletion dramatically decreased biofilm formation in BHI medium and gastric fluid medium, and reduced the invasion and replication rate into the Caco-2BBe cells and RAW264.7 cells. However, complementation restored the wild-type phenotype. Importantly, mouse infection experiments demonstrated that hfq played a more important role in the colonisation and virulence in serotype 4b strain Lm NTSN than in the serotype 1/2a strain Lm EGDe. Taken together, these results demonstrated that hfq is a novel factor associated with biofilm formation, and plays an essential role in the stress response and pathogenisis in serotype 4b strain Lm NTSN. Our data provide the basis for further research into the function of Hfq in serotype 4b Listeria monocytogenes.



中文翻译:

hfq在血清型4b单核细胞增生李斯特菌中参与生物膜形成和毒力的重要作用

在革兰氏阳性和革兰氏阴性细菌中都广泛检测到调节因子Hfq。然而,它在革兰氏阳性细菌中的作用尚不明确,并且在物种之间有所不同。在李斯特菌Lm的),能够适应各种环境和生活都saprobiotic和寄生生活方式的有机体,HFQ的作用尚不完全清楚。血清型4b单核细胞增生李斯特菌菌株与大多数李斯特菌病暴发有关,而hfq在血清型4b菌株中的功能仍未提及。在这里,我们构建了血清型4b Lm NTSN的hfq缺失和回复突变体,并在体外分析了它们的生物学特性体内hfq的缺失导致在含有4.5%乙醇或1%Triton X-100的培养基中缺乏生长,并且突变体的生长在4°C时明显减少。此外,hfq缺失显着减少了BHI培养基和胃液培养基中生物膜的形成,并降低了其对Caco-2BBe细胞和RAW264.7细胞的侵袭和复制速率。然而,互补恢复了野生型表型。重要的是,小鼠感染实验证实HFQ在定植起到更重要的作用和毒力在血清型4b中应变Lm的NTSN比在血清型1 / 2a中应变Lm的菌EGDe。综上所述,这些结果表明,hfq是与生物膜形成相关的新型因子,并且在血清型4b菌株Lm NTSN的应激反应和致病性中起重要作用。我们的数据为进一步研究Hfq在4b型李斯特菌血清型中的功能提供了基础

更新日期:2018-07-07
down
wechat
bug