Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63 V vs. SHE), the current production capabilities of two Shewanella strains—NTOU1 and KR-12—were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (Q_TCA) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (Q_EL) and Q_TCA with an R² of 0.65. In addition, a large amount of pyruvate accumulation was observed at pH = 6, rendering Q_TCA negative. The results indicate an occurrence of an undesired cataplerotic reaction. It was also found that Q_TCA provides important information showing the oxygen-boosting TCA cycle and anodic-current generation of Shewanella spp. Linear dependence of the change in charge for biomass growth (4.52FΔn_Cell) on Q_TCA was also found as expressed by 4.52FΔn_Cell = 1.0428 Q_TCA + 0.0442, indicating that these two charge quantities are inherently identical under most of the experimental conditions. In the mediator-spiked experiments, the external addition of the mediators (ferricyanide, anthraquinone-2, 6-disulfonate, and riboflavin) beyond certain concentrations inhibited the activity of the TCA cycle, indicating that the oxidative phosphorylation is deactivated by excessive amounts of mediators, yet Shewanella spp. are constrained with regard to carrying out the substrate-level phosphorylation.

使用配备有碳毡电极的电化学电池（相对于SHE保持+0.63 V的平衡），在各种条件下使用乳酸作为电子供体，检验了两种希瓦氏菌菌株NTOU1和KR-12的当前生产能力。通过质量平衡计算在三羧酸循环中产生的代谢电荷（Q _TCA）。数据表明所述电动产生的库仑（之间的线性关系Q _EL）和Q _TCA与[R ²为0.65。此外，在pH = 6时观察到大量丙酮酸积累，从而使Q _TCA升高。消极的。结果表明发生了不希望的僵化反应。还发现Q _TCA提供了重要的信息，显示了希瓦氏菌属的增氧TCA循环和阳极电流的产生。用于生物量生长（4.52中电荷的改变的线性相关˚F Δ Ñ_细胞）上Q _TCA还发现由4.52表示˚F Δ Ñ_细胞 = 1.0428 Q _TCA + 0.0442，表明这两个电荷量在大多数实验条件下本质上是相同的。在加标介体的实验中，超过一定浓度的外部添加介体（铁氰化物，蒽醌-2、6-二磺酸盐和核黄素）会抑制TCA循环的活性，表明氧化磷酸化会因过量的介体而失活，但希瓦氏菌属。在进行底物水平的磷酸化方面受到限制。