The uptake of a large variety of contaminants of emerging concern (CECs) by crops has already been reported, and the occurrence of phase II metabolites or conjugates has only been detected in plant cell cultures. However, the extent of their formation under cropping conditions is largely unknown. In this study, an analytical strategy to assess the conjugation of 11 CECs in lettuce (Lactuca sativa L.) irrigated with different concentrations (0, 0.05, 0.5, 5, and 50 μg L⁻¹) of CECs was developed. The methodology involved enzymatic digestion with β-glucosidase to obtain the total fraction (free form + conjugates) of CECs. The conjugation fraction was then obtained based on the difference. The highest extent of conjugation (i.e., 27 to 83%) was found with the most hydrophobic compounds, such as bisphenol A, carbamazepine, methyl paraben, and triclosan. So, the CEC conjugate fraction cannot be neglected in the estimate of human daily intake.

已经报道了农作物对各种新兴污染物（CEC）的吸收，并且仅在植物细胞培养物中检测到了II期代谢产物或结合物的出现。但是，在种植条件下它们的形成程度基本上是未知的。在这项研究中，一种分析策略评估了用不同浓度（0、0.05、0.5、5和50μgL ^-1灌溉）的莴苣（Lactuca sativa L.）中11种CEC的结合。）的CEC。该方法包括用β-葡萄糖苷酶进行酶消化，以获得CEC的总馏分（游离形式+结合物）。然后根据该差异获得结合分数。发现疏水性最高的化合物（例如双酚A，卡马西平，对羟基苯甲酸甲酯和三氯生）的结合程度最高（即27％至83％）。因此，在人类每日摄入量的估计中不能忽略CEC共轭分数。