There is an increasing need for portable and low-cost diagnostic devices for detecting inflammatory/infectious diseases in a rapid and user-friendly fashion. Here, we present a lab-on-a-disc solution, which performs automated sample pre-treatment and combinedly detects small molecules and counts cells in a whole blood sample with a volume of 8.75 μL with a sample to answer time of 14 min. It is used to detect two common inflammation/infection biomarkers, C-reactive protein (CRP) and peripheral blood mononuclear cell (PBMC) count. The whole blood sample was separated into plasma and PBMC fractions using density gradient centrifugation and centrifugo-pneumatic valving. On-disc CRP detection was performed in the extracted plasma using a CRP-antibody-functionalized magnetic nanobead (MNB)-based agglutination assay and a Blu-ray-based optomagnetic detection unit. On-disc PBMC scanning and quantification was performed using an optical imaging unit. Both detection units were integrated on the centrifugal platform and the entire study was automated in order to ensure reliability of the assay and user-friendliness of the method. We measured the CRP level of subjects with different CRP levels and obtained approximately 73% PBMC extraction efficiency compared to hospital results. The concurrent/combined detection of these two common biomarkers in an automated microfluidic platform with integrated detection units and with a low sample-to-answer time is a significant step forward towards a low-cost, out-of-lab, and portable tool to detect multiple biomarkers of significantly different nature (molecules and cells).

越来越需要便携式和低成本的诊断设备，以快速和用户友好的方式检测炎症/感染性疾病。在这里，我们介绍了一种光盘实验室解决方案，该解决方案执行自动样品预处理，并联合检测小分子，并对全血样品中的细胞进行计数，样品的体积为8.75μL，响应时间为14分钟。它用于检测两种常见的炎症/感染生物标志物：C反应蛋白（CRP）和外周血单核细胞（PBMC）计数。使用密度梯度离心和离心风动阀将全血样品分离为血浆和PBMC馏分。使用基于CRP抗体功能化的磁性纳米珠（MNB）的凝集测定和基于蓝光的光检测单元，在提取的血浆中进行盘上CRP检测。使用光学成像单元执行盘上PBMC扫描和定量。两个检测单元均集成在离心平台上，整个研究过程实现了自动化，以确保测定的可靠性和方法的用户友好性。我们测量了具有不同CRP水平的受试者的CRP水平，与医院结果相比，获得了大约73％的PBMC提取效率。在具有集成检测单元且样品到答案时间短的自动化微流控平台中，同时/组合检测这两种常见的生物标志物是迈向低成本实验室外研究的重要一步，