当前位置: X-MOL 学术Microchim. Acta › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Colorimetric determination of ofloxacin using unmodified aptamers and the aggregation of gold nanoparticles
Microchimica Acta ( IF 5.7 ) Pub Date : 2018-07-01 , DOI: 10.1007/s00604-018-2895-2
Xiaotong Zhou , Lumei Wang , Guoqing Shen , Dongwei Zhang , Jialun Xie , Abdureyim Mamut , Weiwen Huang , Shanshan Zhou

AbstractA colorimetric method is presented for the determination of the antibiotic ofloxacin (OFL) in aqueous solution. It is based on the use of an aptamer and gold nanoparticles (AuNPs). In the absence of OFL, the AuNPs are wrapped by the aptamer and maintain dispersed even at the high NaCl concentrations. The solution with colloidally dispersed AuNPs remains red and has an absorption peak at 520 nm. In the presence of OFL, it will bind to the aptamer which is then released from the AuNPs. Hence, AuNPs will aggregate in the salt solution, and color gradually turns to blue, with a new absorption peak at 650 nm. This convenient and specific colorimetric assay for OFL has a linear response in the 20 to 400 nM OFL concentration range and a 3.4 nM detection limit. The method has a large application potential for OFL detection in environmental and biological samples. Graphical abstractSchematic of a sensitive and simple colorimetric aptasensor for ofloxacin (OFL) detection in tap water and synthesic urine. The assay is based on the salt-induced aggregation of gold nanoparticles which results in a color change from red to purple.

中文翻译:

使用未修饰的适体和金纳米粒子的聚集比色测定氧氟沙星

摘要 提出了一种测定水溶液中抗生素氧氟沙星(OFL)的比色法。它基于适体和金纳米粒子 (AuNPs) 的使用。在没有 OFL 的情况下,AuNP 被适体包裹,即使在高 NaCl 浓度下也能保持分散。含有胶体分散的 AuNPs 的溶液保持红色,并在 520 nm 处有一个吸收峰。在 OFL 存在下,它将与适体结合,然后从 AuNP 中释放出来。因此,AuNPs 将在盐溶液中聚集,颜色逐渐变为蓝色,在 650 nm 处出现新的吸收峰。这种方便且特异的 OFL 比色测定在 20 至 400 nM OFL 浓度范围内具有线性响应,检测限为 3.4 nM。该方法在环境和生物样品中的OFL检测中具有很大的应用潜力。用于检测自来水和合成尿液中氧氟沙星 (OFL) 的灵敏且简单的比色适体传感器的图形摘要示意图。该测定基于盐诱导的金纳米颗粒聚集,导致颜色从红色变为紫色。
更新日期:2018-07-01
down
wechat
bug