Electrical stimulation (ES) and magnetic stimulation (MS) can promote bone tissue formation in vivo. Loading ES and MS simultaneously would be very beneficial for bone tissue construction in vitro or in vivo. Magnetic-conductive bi-functional scaffolds which are favorable for the transfer of ES and MS, could further facilitate bone cell/tissue growth. Poly(3,4-ethylenedioxythiophene) (PEDOT)/Fe₃O₄/polylactic acid-co-glycolic acid (PLGA) magnetic-conductive bi-functional fibrous scaffolds were prepared through in situ polymerization of EDOT on Fe₃O₄/PLGA fibers. MC3T3-E1 pre-osteoblasts were incubated on the PEDOT/Fe₃O₄/PLGA fibrous scaffolds and were stimulated by electrical, magnetic and electrical–magnetic signals respectively to detect the impact of different stimulation on cell viability. The measured results show that the scaffolds possess good conductivity and superparamagnetic responsiveness. Furthermore, both electrical and magnetic stimulation promoted cell proliferation and magnetic stimulation could induce cell alignment arrangement. Meanwhile, under electrical–magnetic double stimulation, cell viability was much higher than for cells under single electrical or magnetic stimulation. The growth promoting effects of PEDOT/Fe₃O₄/PLGA fibrous scaffolds under electrical–magnetic double stimulation has great practical potential for bone tissue engineering.

中文翻译：

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基于PEDOT / Fe ₃ O ₄ / PLGA导磁双功能支架的 电磁双重刺激对成骨细胞生长的促进作用

电刺激（ES）和磁刺激（MS）可以促进体内骨骼组织的形成。同时加载ES和MS对体外或体内骨组织的构造将非常有益。有利于ES和MS的转移的导电双功能支架可以进一步促进骨细胞/组织的生长。EDOT在Fe ₃ O ₄上原位聚合制备了聚（3,4-乙撑二氧噻吩）/ Fe ₃ O ₄ /聚乳酸-共乙醇酸（PLGA）导电双功能纤维支架。/ PLGA纤维。将MC3T3-E1前成骨细胞在PEDOT / Fe ₃ O ₄ / PLGA纤维支架上孵育，分别用电，磁和电磁信号刺激，以检测不同刺激对细胞存活力的影响。测量结果表明该支架具有良好的导电性和超顺磁性响应性。此外，电刺激和磁刺激均促进细胞增殖，而磁刺激可诱导细胞排列排列。同时，在电磁双重刺激下，细胞活力远高于在单一电磁或磁性刺激下的细胞活力。PEDOT / Fe ₃ O ₄的生长促进作用电磁双重刺激的/ PLGA纤维支架在骨组织工程中具有巨大的实践潜力。