A reliable, selective and rapid multiclass method has been developed for the simultaneous determination of 55 antibacterial drug residues in shrimp muscle samples by ultra high performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise of eight different classes, namely fluoroquinolones, sulfonamides and synergistic agents, tetracyclines, macrolides, lincosamides, penicillins, nitroimidazole and amphenicols. A simple liquid extraction procedure was developed consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. Chromatographic conditions were optimized, obtaining a running time <10 min. Mean recoveries ranged from 74.3% to 113.3%. For precision test, relative standard deviations (RSD, %) were lower than 15.0% and 24.0% for repeatability and reproducibility, respectively. Limits of detection and quantification ranged from 1.0 to 5.0 ng/g and 3.0-10.0 ng/g, respectively. Finally, the method was applied to real samples and the results demonstrated that enrofloxacin, ciprofloxacin, pefloxacin and doxycycline were quantifiable in shrimp samples.

中文翻译：

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超高效液相色谱-串联质谱法对虾肌肉中抗菌药物的多类分析

建立了一种可靠、选择性、快速的多分类方法，用于超高效液相色谱-串联质谱法同时测定虾肌肉样品中的 55 种抗菌药物残留。研究的化合物包括八种不同的类别，即氟喹诺酮类、磺胺类和增效剂、四环素类、大环内酯类、林可酰胺类、青霉素类、硝基咪唑类和苯酚类。开发了一种简单的液体萃取程序，包括用乙腈和乙二胺四乙酸 (EDTA) 的混合物萃取，然后用正己烷进行脱脂步骤。色谱条件经过优化，运行时间<10 min。平均回收率为 74.3% 至 113.3%。对于精密度测试，相对标准偏差 (RSD, %) 低于 15.0% 和 24。重复性和再现性分别为 0%。检测限和定量限分别为 1.0 至 5.0 ng/g 和 3.0-10.0 ng/g。最后，将该方法应用于实际样品，结果表明虾样品中的恩诺沙星、环丙沙星、培氟沙星和多西环素是可定量的。