Abstract With the increasing focus on the food safety, rapid methods for the detection of Salmonella are crucial for both food industry and regulatory agencies. Recently, many molecular methodologies with diverse technologies have been introduced. Roka Atlas® Salmonella Assay (SEN) is a molecular method that uses ribosomal RNA as target for detection, which is theoretically more sensitive than PCR or isothermal amplification methods that target the DNA sequences of single genes. In this study, SEN assay was compared with four PCR- and isothermal amplification-based assays and a culture method, such as the MicroSEQ® Salmonella spp. Detection kit (MicroSEQ), 3M™ Molecular Detection Assay (MDA) Salmonella, ANSRTM Salmonella Assay (ANSR), and Pro-AmpRTTM SALM spp. Kit (Pro-AmpRT). Food samples were prepared and analyzed according to the current U. S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Salmonella culture method. A total of 155 bacterial isolates (121 for Salmonella inclusivity and 34 for Salmeonlla exclusivity) and 200 egg product samples inoculated at a level of 1–5 CFU/25 g were analyzed. The study also estimated the limit of detection of these molecular methods, and illustrated their advantages and disadvantages. For exclusivity, all 34 non-Salmonella isolates were negative by all 5 molecular methods studied. For inclusivity, all 121 Salmonella isolates were positive by MDA, ANSR, and Pro-AmpRT methods. However, the SEN and MicroSEQ results were negative for 9 samples inoculated with Salmonella bongori. The detection limit of the 5 molecular methods ranged from 1.76 to 3.76 log CFU/mL pre-enrichment culture, with the SEN assay being the most sensitive (1.76 – 2.64 log CFU/mL). The results indicated that the SEN assay was as effective and sensitive in detecting Salmonella enterica in egg products as was the FDA BAM culture method and the 4 other isothermal amplification and PCR methods evaluated in the study.

中文翻译：

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与培养方法、实时 PCR 和等温扩增试验相比，Roka Atlas 沙门氏菌方法检测蛋制品中的沙门氏菌的评价

摘要 随着对食品安全的日益关注，快速检测沙门氏菌的方法对食品工业和监管机构都至关重要。最近，已经引入了许多具有不同技术的分子方法。Roka Atlas® 沙门氏菌检测 (SEN) 是一种以核糖体 RNA 作为检测目标的分子方法，理论上比针对单个基因 DNA 序列的 PCR 或等温扩增方法更灵敏。在本研究中，将 SEN 检测与四种基于 PCR 和等温扩增的检测以及一种培养方法（例如 MicroSEQ® 沙门氏菌属）进行了比较。检测试剂盒 (MicroSEQ)、3M™ 分子检测分析 (MDA) 沙门氏菌、ANSRTM 沙门氏菌分析 (ANSR) 和 Pro-AmpRTTM SALM spp。试剂盒（Pro-AmpRT）。食品样品的制备和分析依据美国现行标准 食品和药物管理局 (FDA) 细菌学分析手册 (BAM) 沙门氏菌培养方法。共分析了 155 个细菌分离株（121 个沙门氏菌和 34 个沙门氏菌排他性）和 200 个以 1–5 CFU/25 g 水平接种的蛋制品样品。该研究还估计了这些分子方法的检测限，并说明了它们的优缺点。就排他性而言，所有 34 种非沙门氏菌分离株均通过所研究的所有 5 种分子方法呈阴性。就包容性而言，所有 121 株沙门氏菌分离株均通过 MDA、ANSR 和 Pro-AmpRT 方法呈阳性。然而，接种沙门氏菌的 9 个样品的 SEN 和 MicroSEQ 结果为阴性。5 种分子方法的检测限范围为 1.76 至 3.76 log CFU/mL 预富集培养，SEN 检测最灵敏（1.76 – 2.64 log CFU/mL）。结果表明，与 FDA BAM 培养方法和研究中评估的其他 4 种等温扩增和 PCR 方法一样，SEN 测定法在检测蛋制品中的肠道沙门氏菌方面同样有效和灵敏。