Molecular-stream separation (MSS), for example, by free flow electrophoresis or continuous annular chromatography, has great potential for applications that require continuous downstream separation such continuous flow synthesis. Despite its potential, MSS still needs to be greatly advanced, which requires currently lacking tools for quantitative characterization of streams in MSS. We developed and introduce here an analytical toolbox for this task. The first tool is a method to convolute 3D raw MSS data into a 2D “angulagram” via signal integration over the whole separation zone using a polar coordinate system. The second tool is three quantitative parameters characterizing stream width, linearity, and deflection, which are determined from an angulagram. The third tool is the analysis of the three parameters in relation to physicochemical characteristics of MSS which reveals deficiencies and guides improvements in MSS devices and methods. Examples of toolbox application to validation of previously published MSS data are provided.

中文翻译：

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分子流分离的定量表征

例如，通过自由流电泳或连续环形色谱法进行的分子流分离（MSS），对于需要连续下游分离（例如连续流合成）的应用具有巨大的潜力。尽管具有潜力，但MSS仍需要大大提高，这要求当前缺乏用于MSS中流的定量表征的工具。我们开发并在此介绍了用于此任务的分析工具箱。第一种工具是使用极坐标系统通过整个分离区域上的信号积分将3D原始MSS数据卷积为2D“角度图”的方法。第二个工具是表征流的宽度，线性和挠度的三个定量参数，这些参数是从一个角度图确定的。第三个工具是分析与MSS的理化特性相关的三个参数，这揭示了缺陷并指导MSS装置和方法的改进。提供了用于验证先前发布的MSS数据的工具箱应用程序示例。